Outbreak investigation of acute febrile illness from the Himalayan foothills: Solving the puzzle of fever

Frontiers in Pharmacology

Pharmacology

Kapil Goel¹  Arun Aggarwal¹  Radha Kanta Ratho²  Subhabrata Sarkar²  Kanwalpreet Kaur²  Vikrant Sharma²  Prakasini Satapathy²  Mannat Kang²  Shefali Dhingra²  Ishani Bora²  Neeraj Arora³ 

[1] Department of Community Medicine & SPH, PGIMER, Chandigarh, India;Department of Virology, Postgraduate Institute of Medical Education and Research, Chandigarh, India;Lab Medicine, Civil Hospital, Panchkula, India;

关键词: acute febrile illness;    dengue fever;    DENV-2;    cosmopoliton genotype;    outbreak;   

DOI  :  10.3389/fphar.2023.1159377

 received in 2023-02-05, accepted in 2023-03-30,  发布年份 2023

来源: Frontiers

【 摘 要 】

In September 2022, Panchkula Civil Hospital reported an outbreak of acute febrile illness (AFI) in Pinjore, located in the Himalayan foothills, Haryana, North India. There was an upsurge of fever cases. Blood samples were taken from suspected patients (n = 58) with AFI and subjected to serology of dengue, chikungunya, Japanese encephalitis, leptospira and scrub typhus. The samples were also screened for West Nile & Zika virus RNA using real-time PCR. Viral strains were characterized by sequencing. Of the 58 cases of AFI, Dengue could be identified in 45 (77.58%) followed by JE and Chikungunya in 2 cases each (3.44%), respectively. Among Dengue positive cases, 44 had monoinfection (97.77%) and 1 patient had dengue and JE. None were positive for Zika, West Nile, Scrub typhus, and Leptospira with the testing protocol. Four patients developed dengue with warning signs, such as abdominal pain in one patient and recurrent vomiting in the remaining three. The dengue serotype could be determined in 17 samples and revealed serotype 2. Molecular evolution analysis based on the complete envelope gene revealed that all DENV-2 strains (n = 13) circulated in the outbreak area belonged to the DENV-2 cosmopoliton genotype. In the early stages of infection, relying only on clinical manifestations is ineffective, so both molecular and serological assays along with clinical diagnosis are noteworthy for determining the aetiology of AFI.

【 授权许可】

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Copyright © 2023 Satapathy, Goel, Sharma, Sarkar, Kang, Dhingra, Bora, Kaur, Arora, Aggarwal and Ratho.