Frontiers in Immunology | |
T cell Ca2+ microdomains through the lens of computational modeling | |
Immunology | |
Andreas H. Guse1  Diana C. Gil Montoya1  Björn-Philipp Diercks1  Roberto Ornelas-Guevara2  Geneviève Dupont2  | |
[1] The Calcium Signalling Group, Department of Biochemistry and Molecular Cell Biology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany;Unit of Theoretical Chronobiology, Faculté des Sciences CP231, Université Libre de Bruxelles (ULB), Brussels, Belgium; | |
关键词: store-operated Ca entry (SOCE); Orai; 3D computational model; Ca microdomains; nicotinic acid adenine dinucleotide phosphate (NAADP); | |
DOI : 10.3389/fimmu.2023.1235737 | |
received in 2023-06-06, accepted in 2023-09-18, 发布年份 2023 | |
来源: Frontiers | |
【 摘 要 】
Cellular Ca2+ signaling is highly organized in time and space. Locally restricted and short-lived regions of Ca2+ increase, called Ca2+ microdomains, constitute building blocks that are differentially arranged to create cellular Ca2+ signatures controlling physiological responses. Here, we focus on Ca2+ microdomains occurring in restricted cytosolic spaces between the plasma membrane and the endoplasmic reticulum, called endoplasmic reticulum-plasma membrane junctions. In T cells, these microdomains have been finely characterized. Enough quantitative data are thus available to develop detailed computational models of junctional Ca2+ dynamics. Simulations are able to predict the characteristics of Ca2+ increases at the level of single channels and in junctions of different spatial configurations, in response to various signaling molecules. Thanks to the synergy between experimental observations and computational modeling, a unified description of the molecular mechanisms that create Ca2+ microdomains in the first seconds of T cell stimulation is emerging.
【 授权许可】
Unknown
Copyright © 2023 Gil Montoya, Ornelas-Guevara, Diercks, Guse and Dupont
【 预 览 】
Files | Size | Format | View |
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RO202311145660205ZK.pdf | 1689KB | download |