Unravelling the sexual developmental biology of Cystoisospora suis, a model for comparative coccidian parasite studies

Frontiers in Cellular and Infection Microbiology

Cellular and Infection Microbiology

Teresa Cruz-Bustos¹  Anja Joachim¹  Anna Sophia Feix¹  Bärbel Ruttkowski¹  Marlies Dolezal²  Ebrahim Razzazi-Fazeli³  Karin Hummel³ 

[1] Department of Pathobiology, Institute of Parasitology, University of Veterinary Medicine Vienna, Vienna, Austria;Platform for Bioinformatics and Biostatistics, Department of Biomedical Sciences, University of Veterinary Medicine Vienna, Vienna, Austria;VetCore Facility (Proteomics), University of Veterinary Medicine Vienna, Vienna, Austria;

关键词: Isospora suis;    coccidia;    Apicomplexa;    sexual development;    proteome;    metabolism;    oocyst;    gamonts;   

DOI  :  10.3389/fcimb.2023.1271731

 received in 2023-08-02, accepted in 2023-09-28,  发布年份 2023

来源: Frontiers

【 摘 要 】

IntroductionThe apicomplexan parasite Cystoisospora suis has global significance as an enteropathogen of suckling piglets. Its intricate life cycle entails a transition from an asexual phase to sexual development, ultimately leading to the formation of transmissible oocysts.MethodsTo advance our understanding of the parasite’s cellular development, we complemented previous transcriptome studies by delving into the proteome profiles at five distinct time points of in vitro cultivation through LC/MS-MS analysis.ResultsA total of 1,324 proteins were identified in the in vitro developmental stages of C. suis, and 1,082 proteins were identified as significantly differentially expressed. Data are available via ProteomeXchange with identifier PXD045050. We performed BLAST, GO enrichment, and KEGG pathway analyses on the up- and downregulated proteins to elucidate correlated events in the C. suis life cycle. Our analyses revealed intriguing metabolic patterns in macromolecule metabolism, DNA- and RNA-related processes, proteins associated with sexual stages, and those involved in cell invasion, reflecting the adaptation of sexual stages to a nutrient-poor and potentially stressful extracellular environment, with a focus on enzymes involved in metabolism and energy production.DiscussionThese findings have important implications for understanding the developmental biology of C. suis as well as other, related coccidian parasites, such as Eimeria spp. and Toxoplasma gondii. They also support the role of C. suis as a new model for the comparative biology of coccidian tissue cyst stages.

【 授权许可】

Unknown   
Copyright © 2023 Cruz-Bustos, Dolezal, Feix, Ruttkowski, Hummel, Razzazi-Fazeli and Joachim