Yield, nutrition, and leaf gas exchange of lettuce plants in a hydroponic system in response to Bacillus subtilis inoculation

Frontiers in Plant Science

Plant Science

Khaloud Mohammed Alarjani¹  Mostafa A. Abdel-Maksoud¹  Jailson Vieira Aguilar²  Liliane Santos de Camargos²  Tiago Zoz³  Marcelo Carvalho Minhoto Teixeira Filho⁴  Carlos Eduardo da Silva Oliveira⁴  Arshad Jalal⁴  Bhim Bahadur Ghaley⁵  Hamada AbdElgawad⁶ 

[1] Botany and Microbiology Department, College of Science, King Saud University, Riyadh, Saudi Arabia;Department of Biology and Zootechnics, Lab of Plant Morphology and Anatomy/Lab Plant Metabolism and Physiology, School of Engineering, São Paulo State University - UNESP-FEIS, Ilha Solteira, São Paulo, Brazil;Department of Crop Science, State University of Mato Grosso do Sul – UEMS, Mundo Novo, Mato Grosso do Sul, Brazil;Department of Plant Protection, Rural Engineering and Soils, School of Engineering, São Paulo State University - UNESP-FEIS, Ilha Solteira, São Paulo, Brazil;Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Copenhagen, Denmark;Laboratory for Molecular Plant Physiology and Biotechnology, Department of Biology, University of Antwerp, Antwerp, Belgium;

关键词: biological nitrogen fixation;    growth-promoting bacteria;    Lactuca sativa;    photosynthetic efficiency;    water use efficiency;   

DOI  :  10.3389/fpls.2023.1248044

 received in 2023-06-26, accepted in 2023-10-06,  发布年份 2023

来源: Frontiers

【 摘 要 】

Inoculation with Bacillus subtilis is a promising approach to increase plant yield and nutrient acquisition. In this context, this study aimed to estimate the B. subtilis concentration that increases yield, gas exchange, and nutrition of lettuce plants in a hydroponic system. The research was carried out in a greenhouse in Ilha Solteira, Brazil. A randomized block design with five replications was adopted. The treatments consisted of B. subtilis concentrations in nutrient solution [0 mL “non-inoculated”, 7.8 × 103, 15.6 × 103, 31.2 × 103, and 62.4 × 103 colony forming units (CFU) mL−1 of nutrient solution]. There was an increase of 20% and 19% in number of leaves and 22% and 25% in shoot fresh mass with B. subtilis concentrations of 15.6 × 103 and 31.2 × 103 CFU mL−1 as compared to the non-inoculated plants, respectively. Also, B. subtilis concentration at 31.2 × 103 CFU mL−1 increased net photosynthesis rate by 95%, intercellular CO2 concentration by 30%, and water use efficiency by 67% as compared to the non-inoculated treatments. The concentration of 7.8 × 103 CFU mL−1 improved shoot accumulation of Ca, Mg, and S by 109%, 74%, and 69%, when compared with non-inoculated plants, respectively. Inoculation with B. subtilis at 15.6 × 103 CFU mL−1 provided the highest fresh leaves yield while inoculation at 15.6 × 103 and 31.2 × 103 CFU mL−1 increased shoot fresh mass and number of leaves. Concentrations of 7.8 × 103 and 15.6 × 103 increased shoot K accumulation. The concentrations of 7.8 × 103, 15.6 × 103, and 31.2 × 103 CFU mL−1 increased shoot N accumulation in hydroponic lettuce plants.

【 授权许可】

Unknown   
Copyright © 2023 Oliveira, Jalal, Aguilar, de Camargos, Zoz, Ghaley, Abdel-Maksoud, Alarjani, AbdElgawad and Teixeira Filho