The ability to induce heat shock transcription factor-regulated genes in response to lethal heat stress is associated with thermotolerance in tomato cultivars

Frontiers in Plant Science

Plant Science

Satoshi Kidokoro¹  Junya Mizoi¹  Ken-Suke Kodaira¹  Daisuke Todaka¹  Tomohiro Imatomi¹  Kazuko Yamaguchi-Shinozaki²  Kazuo Shinozaki³  Tetsuya Sakurai⁴  Hidehito Takayama⁵ 

[1] Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan;Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan;Research Institute for Agricultural and Life Sciences, Tokyo University of Agriculture, Tokyo, Japan;Gene Discovery Research Group, RIKEN Center for Sustainable Resource Science, Tsukuba, Japan;Institute for Advanced Research, Nagoya University, Nagoya, Japan;RIKEN Center for Sustainable Resource Science, Yokohama, Japan;Interdisciplinary Science Unit, Multidisciplinary Science Cluster, Research and Education Faculty, Kochi University, Nankoku, Japan;The KAITEKI Institute, Inc., Tokyo, Japan;

关键词: heat stress;    Solanum lycopersicum;    thermotolerance;    cultivars;    expression biomarker;    heat shock response;    transcriptome;   

DOI  :  10.3389/fpls.2023.1269964

 received in 2023-07-31, accepted in 2023-09-18,  发布年份 2023

来源: Frontiers

【 摘 要 】

Heat stress is a severe challenge for plant production, and the use of thermotolerant cultivars is critical to ensure stable production in high-temperature-prone environments. However, the selection of thermotolerant cultivars is difficult due to the complex nature of heat stress and the time and space needed for evaluation. In this study, we characterized genome-wide differences in gene expression between thermotolerant and thermosensitive tomato cultivars and examined the possibility of selecting gene expression markers to estimate thermotolerance among different tomato cultivars. We selected one thermotolerant and one thermosensitive cultivar based on physiological evaluations and compared heat-responsive gene expression in these cultivars under stepwise heat stress and acute heat shock conditions. Transcriptomic analyses reveled that two heat-inducible gene expression pathways, controlled by the heat shock element (HSE) and the evening element (EE), respectively, presented different responses depending on heat stress conditions. HSE-regulated gene expression was induced under both conditions, while EE-regulated gene expression was only induced under gradual heat stress conditions in both cultivars. Furthermore, HSE-regulated genes showed higher expression in the thermotolerant cultivar than the sensitive cultivar under acute heat shock conditions. Then, candidate expression biomarker genes were selected based on the transcriptome data, and the usefulness of these candidate genes was validated in five cultivars. This study shows that the thermotolerance of tomato is correlated with its ability to maintain the heat shock response (HSR) under acute severe heat shock conditions. Furthermore, it raises the possibility that the robustness of the HSR under severe heat stress can be used as an indicator to evaluate the thermotolerance of crop cultivars.

【 授权许可】

Unknown   
Copyright © 2023 Mizoi, Todaka, Imatomi, Kidokoro, Sakurai, Kodaira, Takayama, Shinozaki and Yamaguchi-Shinozaki