Frontiers in Neuroscience | |
Short-term memory impairment following recovery from systemic inflammation induced by lipopolysaccharide in mice | |
Neuroscience | |
Shu Watanuki1  Kohei Morimoto1  Ryota Eguchi1  Ken-ichi Otsuguro1  Taisuke Kitano2  | |
[1] Laboratory of Pharmacology, Department of Basic Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan;Laboratory of Veterinary Biochemistry, School of Veterinary Medicine, Kitasato University, Aomori, Japan; | |
关键词: lipopolysaccharide; neuroinflammation; dendritic spine; memory; astrocyte; microglia; hippocampus; gliosis; | |
DOI : 10.3389/fnins.2023.1273039 | |
received in 2023-08-05, accepted in 2023-09-27, 发布年份 2023 | |
来源: Frontiers | |
【 摘 要 】
The relationship between neuroinflammation and mental disorders has been recognized and investigated for over 30 years. Diseases of systemic or peripheral inflammation, such as sepsis, peritonitis, and infection, are associated with increased risk of mental disorders with neuroinflammation. To elucidate the pathogenesis, systemic administration of lipopolysaccharide (LPS) in mice is often used. LPS-injected mice exhibit behavioral abnormalities with glial activation. However, these studies are unlikely to recapitulate the clinical pathophysiology of human patients, as most studies focus on the acute inflammatory response with systemic symptoms occurring within 24 h of LPS injection. In this study, we focus on the effects of LPS on behavioral abnormalities following recovery from systemic symptoms and investigate the mechanisms of pathogenesis. Several behavioral tests were performed in LPS-injected mice, and to assess neuroinflammation, the time course of the morphological change and expression of inflammatory factors in neurons, astrocytes, and microglia were investigated. At 7 days post-LPS injection, mice exhibited short-term memory impairment accompanied by the suppression of neuronal activity and increases in morphologically immature spines. Glial cells were transiently activated in the hippocampus concomitant with upregulation of the microglial phagocytosis marker CD68 3 days after injection. Here we show that transient glial cell activation in the acute response phase affects neuronal activity and behavior following recovery from systemic symptoms. These findings provide novel insights for studies using the LPS-induced inflammation model and that will contribute to the development of treatments for mental disorders of this etiology.
【 授权许可】
Unknown
Copyright © 2023 Morimoto, Watanuki, Eguchi, Kitano and Otsuguro.
【 预 览 】
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RO202311140681260ZK.pdf | 21123KB | download |