Exploring microbial diversity responses in agricultural fields: a comparative analysis under pesticide stress and non-stress conditions

Frontiers in Microbiology

Microbiology

Geeta Bhandari¹  Najat A. Bukhari²  Kahkashan Perveen²  Anita Sharma³  Garima Pant⁴  Ranjana Rani⁵  Saurabh Gangola⁶  Samiksha Joshi⁶ 

[1] Department of Biosciences, Swami Rama Himalayan University, Dehradun, India;Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia;Department of Microbiology, GBPUAT, Pantnagar, India;Department of PDP, Graphic Era Hill University, Bhimtal, India;School of Agriculture and Food Science, University of Queensland, Brisbane, QLD, Australia;School of Agriculture, Graphic Era Hill University, Bhimtal, India;

关键词: pesticides;    biodegradation;    metagenomics;    agriculture;    environment;   

DOI  :  10.3389/fmicb.2023.1271129

 received in 2023-08-01, accepted in 2023-08-30,  发布年份 2023

来源: Frontiers

【 摘 要 】

Exposure to pesticides changes the microbial community structure in contaminated agricultural fields. To analyze the changes in the native microbial composition qRT-PCR, a metagenomic study was conducted. The qRT-PCR results exhibited that the uncontaminated soil has a higher copy number of 16S rDNA relative to the soil contaminated with pesticide. Metagenome analysis interprets that uncontaminated soil is enriched with proteobacteria in comparison with pesticide-contaminated soil. However, the presence of Actinobacteria, Firmicutes, and Bacteroides was found to be dominant in the pesticide-spiked soil. Additionally, the presence of new phyla such as Chloroflexi, Planctomycetes, and Verrucomicrobia was noted in the pesticide-spiked soil, while Acidobacteria and Crenarchaeota were observed to be extinct. These findings highlight that exposure to pesticides on soil significantly impacts the biological composition of the soil. The abundance of microbial composition under pesticide stress could be of better use for the treatment of biodegradation and bioremediation of pesticides in contaminated environments.

【 授权许可】

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Copyright © 2023 Gangola, Joshi, Bhandari, Pant, Sharma, Perveen, Bukhari and Rani.