High confidence QTLs and key genes identified using Meta-QTL analysis for enhancing heat tolerance in chickpea (Cicer arietinum L.)

Frontiers in Plant Science

Plant Science

Rajeev K. Varshney¹  Aakash Sahu²  Shreshth Gupta²  Vinay Kumar Sharma²  Mahendar Thudi³  Sagar Krushnaji Rangari⁴  Raj Kumar⁴  Dinakaran Elango⁵  Sunil S. Gangurde⁶  Reyazul Rouf Mir⁷  Uday Chand Jha⁸  Pooran M. Gaur⁹  Himabindu Kudapa⁹  Pronob J. Paul^1,10 

[1] Centre for Crop & Food Innovation, WA State Agricultural Biotechnology Centre, Food Futures Institute, Murdoch University, Murdoch, WA, Australia;Department of Agricultural Biotechnology and Molecular Biology, Dr. Rajendra Prasad Central Agricultural University (RPCAU), Pusa, Bihar, India;Department of Agricultural Biotechnology and Molecular Biology, Dr. Rajendra Prasad Central Agricultural University (RPCAU), Pusa, Bihar, India;Center for Crop Health, University of Southern Queensland, Toowoomba, QLD, Australia;Department of Agricultural Biotechnology and Molecular Biology, Dr. Rajendra Prasad Central Agricultural University (RPCAU), Pusa, Bihar, India;Research Program-Accelerated Crop Improvement, International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, Telangana, India;Department of Agronomy, Iowa State University, Ames, IA, United States;Department of Plant Pathology, University of Georgia, Tifton, GA, United States;Faculty of Agriculture, Sher-e-Kashmir University of Agricultural Sciences and Technology (SKUAST), Sopore, India;Indian Council for Agricultural Research (ICAR)- Indian Institute of Pulses Research (IIPR), Kanpur, Uttar Pradesh, India;Research Program-Accelerated Crop Improvement, International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, Telangana, India;Research Program-Accelerated Crop Improvement, International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, Telangana, India;Rice Breeding Innovations, International Rice Research Institute (IRRI), South Asia-Hub, Patancheru, Telangana, India;

关键词: candidate genes;    confidence interval;    heat stress;    Meta-QTLs;    recombinant inbred lines;   

DOI  :  10.3389/fpls.2023.1274759

 received in 2023-08-08, accepted in 2023-09-25,  发布年份 2023

来源: Frontiers

【 摘 要 】

The rising global temperatures seriously threaten sustainable crop production, particularly the productivity and production of heat-sensitive crops like chickpeas. Multiple QTLs have been identified to enhance the heat stress tolerance in chickpeas, but their successful use in breeding programs remains limited. Towards this direction, we constructed a high-density genetic map spanning 2233.5 cM with 1069 markers. Using 138 QTLs reported earlier, we identified six Meta-QTL regions for heat tolerance whose confidence interval was reduced by 2.7-folds compared to the reported QTLs. Meta-QTLs identified on CaLG01 and CaLG06 harbor QTLs for important traits, including days to 50% flowering, days to maturity, days to flower initiation, days to pod initiation, number of filled pods, visual score, seed yield per plant, biological yield per plant, chlorophyll content, and harvest index. In addition, key genes identified in Meta-QTL regions like Pollen receptor-like kinase 3 (CaPRK3), Flowering-promoting factor 1 (CaFPF1), Flowering Locus C (CaFLC), Heat stress transcription factor A-5 (CaHsfsA5), and Pollen-specific leucine-rich repeat extensins (CaLRXs) play an important role in regulating the flowering time, pollen germination, and growth. The consensus genomic regions, and the key genes reported in this study can be used in genomics-assisted breeding for enhancing heat tolerance and developing heat-resilient chickpea cultivars.

【 授权许可】

Unknown   
Copyright © 2023 Kumar, Sharma, Rangari, Jha, Sahu, Paul, Gupta, Gangurde, Kudapa, Mir, Gaur, Varshney, Elango and Thudi