Structure, evolution and functional inference on the Mildew Locus O (MLO) gene family in three cultivated Cucurbitaceae spp.

BMC Genomics

Research Article

Giuseppe Andolfo¹  Paolo Iovieno¹  Luigi Frusciante¹  Maria Raffaella Ercolano¹  Luigi Ricciardi²  Stefano Pavan²  Adalgisa Schiavulli³  Domenico Catalano³ 

[1] Department of Agriculture Sciences, University of Naples ‘Federico II’, Via Università 100, 80055, Naples, Portici, Italy;Department of Soil, Plant and Food Science, University of Bari “Aldo Moro”, Via Amendola 165/A, 70126, Bari, Italy;Institute of Biosciences and Bioresources, Italian National Council of Research, Via Amendola 165/A, 70126, Bari, Italy;

关键词: MLO;    Cucurbitaceae;    Evolution;    Powdery mildew;    Resistance;   

DOI  :  10.1186/s12864-015-2325-3

 received in 2015-06-16, accepted in 2015-12-15,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundThe powdery mildew disease affects thousands of plant species and arguably represents the major fungal threat for many Cucurbitaceae crops, including melon (Cucumis melo L.), watermelon (Citrullus lanatus L.) and zucchini (Cucurbita pepo L.). Several studies revealed that specific members of the Mildew Locus O (MLO) gene family act as powdery mildew susceptibility factors. Indeed, their inactivation, as the result of gene knock-out or knock-down, is associated with a peculiar form of resistance, referred to as mlo resistance.ResultsWe exploited recently available genomic information to provide a comprehensive overview of the MLO gene family in Cucurbitaceae. We report the identification of 16 MLO homologs in C. melo, 14 in C. lanatus and 18 in C. pepo genomes. Bioinformatic treatment of data allowed phylogenetic inference and the prediction of several ortholog pairs and groups. Comparison with functionally characterized MLO genes and, in C. lanatus, gene expression analysis, resulted in the detection of candidate powdery mildew susceptibility factors. We identified a series of conserved amino acid residues and motifs that are likely to play a major role for the function of MLO proteins. Finally, we performed a codon-based evolutionary analysis indicating a general high level of purifying selection in the three Cucurbitaceae MLO gene families, and the occurrence of regions under diversifying selection in candidate susceptibility factors.ConclusionsResults of this study may help to address further biological questions concerning the evolution and function of MLO genes. Moreover, data reported here could be conveniently used by breeding research, aiming to select powdery mildew resistant cultivars in Cucurbitaceae.

【 授权许可】

CC BY   
© Iovieno et al. 2015