Aluminum tolerance association mapping in triticale

BMC Genomics

Research Article

Andrzej Kilian¹  Henryk Cichy²  Grzegorz Budzianowski²  Piotr T Bednarek³  Andrzej Anioł³  Agnieszka Niedziela³ 

[1] Diversity Array Technology Pty Ltd, 1 Wilf Crane Crescent, 2600, Yarralumla, ACT, Australia;Plant Breeding Company-Strzelce, Breeding Department Małyszyn, 66-400 Gorzów Wlkp., Myśliborska Str. 81, Poland;Plant Breeding and Acclimatization Institute, 05-870, Błonie, Radzików, Poland;

关键词: Mixed Linear Model;    Association Mapping;    DArT Marker;    Aluminum Tolerance;    Diversity Array Technology;   

DOI  :  10.1186/1471-2164-13-67

 received in 2011-07-13, accepted in 2012-02-13,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundCrop production practices and industrialization processes result in increasing acidification of arable soils. At lower pH levels (below 5.0), aluminum (Al) remains in a cationic form that is toxic to plants, reducing growth and yield. The effect of aluminum on agronomic performance is particularly important in cereals like wheat, which has promoted the development of programs directed towards selection of tolerant forms. Even in intermediately tolerant cereals (i.e., triticale), the decrease in yield may be significant. In triticale, Al tolerance seems to be influenced by both wheat and rye genomes. However, little is known about the precise chromosomal location of tolerance-related genes, and whether wheat or rye genomes are crucial for the expression of that trait in the hybrid.ResultsA mapping population consisting of 232 advanced breeding triticale forms was developed and phenotyped for Al tolerance using physiological tests. AFLP, SSR and DArT marker platforms were applied to obtain a sufficiently large set of molecular markers (over 3000). Associations between the markers and the trait were tested using General (GLM) and Multiple (MLM) Linear Models, as well as the Statistical Machine Learning (SML) approach. The chromosomal locations of candidate markers were verified based on known assignments of SSRs and DArTs or by using genetic maps of rye and triticale.Two candidate markers on chromosome 3R and 9, 15 and 11 on chromosomes 4R, 6R and 7R, respectively, were identified. The r2 values were between 0.066 and 0.220 in most cases, indicating a good fit of the data, with better results obtained with the GML than the MLM approach. Several QTLs on rye chromosomes appeared to be involved in the phenotypic expression of the trait, suggesting that rye genome factors are predominantly responsible for Al tolerance in triticale.ConclusionsThe Diversity Arrays Technology was applied successfully to association mapping studies performed on triticale breeding forms. Statistical approaches allowed the identification of numerous markers associated with Al tolerance. Available rye and triticale genetic maps suggested the putative location of the markers and demonstrated that they formed several linked groups assigned to distinct chromosomes (3R, 4R, 6R and 7R). Markers associated with genomic regions under positive selection were identified and indirectly mapped in the vicinity of the Al-tolerant markers. The present findings were in agreement with prior reports.

【 授权许可】

Unknown   
© Niedziela et al; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.