Re-annotation of the Saccharopolyspora erythraea genome using a systems biology approach

BMC Genomics

Research Article

Robin W Palfreyman¹  Lars K Nielsen¹  Tim R Mercer¹  Esteban Marcellin¹  Cuauhtemoc Licona-Cassani¹ 

[1] Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, 4072, Brisbane, Qld, Australia;

关键词: Proteogenomics;    Saccharopolyspora erythraea;    Systems biology;    Genome annotation;    High G + C content genomes;   

DOI  :  10.1186/1471-2164-14-699

 received in 2013-05-18, accepted in 2013-09-24,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundAccurate bacterial genome annotations provide a framework to understanding cellular functions, behavior and pathogenicity and are essential for metabolic engineering. Annotations based only on in silico predictions are inaccurate, particularly for large, high G + C content genomes due to the lack of similarities in gene length and gene organization to model organisms.ResultsHere we describe a 2D systems biology driven re-annotation of the Saccharopolyspora erythraea genome using proteogenomics, a genome-scale metabolic reconstruction, RNA-sequencing and small-RNA-sequencing. We observed transcription of more than 300 intergenic regions, detected 59 peptides in intergenic regions, confirmed 164 open reading frames previously annotated as hypothetical proteins and reassigned function to open reading frames using the genome-scale metabolic reconstruction. Finally, we present a novel way of mapping ribosomal binding sites across the genome by sequencing small RNAs.ConclusionsThe work presented here describes a novel framework for annotation of the Saccharopolyspora erythraea genome. Based on experimental observations, the 2D annotation framework greatly reduces errors that are commonly made when annotating large-high G + C content genomes using computational prediction algorithms.

【 授权许可】

CC BY   
© Marcellin et al.; licensee BioMed Central Ltd. 2013