Discovery and characterization of medaka miRNA genes by next generation sequencing platform

BMC Genomics

Proceedings

Sung-Chou Li¹  Meng-Ru Ho¹  Wen-Ching Chan²  Wen-chang Lin³  Ling-Yueh Hu⁴  Chun-Hung Lai⁴  Kuo-Wang Tsai⁴  Pung-Pung Hwang⁵  Chun-Nan Hsu⁶ 

[1] Institute of Biomedical Informatics, National Yang-Ming University, Taipei, Taiwan;Bioinformatics Program, Taiwan International Graduate Program, Academia Sinica, Taipei, Taiwan;Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan;Institute of Biomedical Informatics, National Yang-Ming University, Taipei, Taiwan;Bioinformatics Program, Taiwan International Graduate Program, Academia Sinica, Taipei, Taiwan;Institute of Information Sciences, Academia Sinica, Taipei, Taiwan;Institute of Biomedical Informatics, National Yang-Ming University, Taipei, Taiwan;Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan;Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan;Institute of Cellular and Organismic Biology, Academia Sinica, Taiwan;Institute of Information Sciences, Academia Sinica, Taipei, Taiwan;Information Sciences Institute, University of Southern California, 90292, Marina del Rey, CA, USA;

关键词: Next Generation Sequencing;    miRNA Gene;    Mature miRNAs;    Next Generation Sequencing Technology;    miRNA Cluster;   

DOI  :  10.1186/1471-2164-11-S4-S8

来源: Springer

【 摘 要 】

BackgroundMicroRNAs (miRNAs) are endogenous non-protein-coding RNA genes which exist in a wide variety of organisms, including animals, plants, virus and even unicellular organisms. Medaka (Oryzias latipes) is a useful model organism among vertebrate animals. However, no medaka miRNAs have been investigated systematically. It is beneficial to conduct a genome-wide miRNA discovery study using the next generation sequencing (NGS) technology, which has emerged as a powerful sequencing tool for high-throughput analysis.ResultsIn this study, we adopted ABI SOLiD platform to generate small RNA sequence reads from medaka tissues, followed by mapping these sequence reads back to medaka genome. The mapped genomic loci were considered as candidate miRNAs and further processed by a support vector machine (SVM) classifier. As result, we identified 599 novel medaka pre-miRNAs, many of which were found to encode more than one isomiRs. Besides, additional minor miRNAs (also called miRNA star) can be also detected with the improvement of sequencing depth. These quantifiable isomiRs and minor miRNAs enable us to further characterize medaka miRNA genes in many aspects. First of all, many medaka candidate pre-miRNAs position close to each other, forming many miRNA clusters, some of which are also conserved across other vertebrate animals. Secondly, during miRNA maturation, there is an arm selection preference of mature miRNAs within precursors. We observed the differences on arm selection preference between our candidate pre-miRNAs and their orthologous ones. We classified these differences into three categories based on the distribution of NGS reads. Finally, we also investigated the relationship between conservation status and expression level of miRNA genes. We concluded that the evolutionally conserved miRNAs were usually the most abundant ones.ConclusionsMedaka is a widely used model animal and usually involved in many biomedical studies, including the ones on development biology. Identifying and characterizing medaka miRNA genes would benefit the studies using medaka as a model organism.

【 授权许可】

Unknown   
© Li et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.