Hybrid capture vs. PCR screening of cervical human papilloma virus infections. Cytological and histological associations in 1270 women

BMC Cancer

Research Article

Zanis Voulgaris¹  John Panayiotides²  Sotirios Tsiodras³  Angeliki Tsivilika⁴  John Georgoulakis⁵  Petros Karakitsos⁶  Aikaterini Chranioti⁶  Amanda Psyrri⁷ 

[1] 1st Department of Obstetrics and Gynecology, University of Athens Med. Sch. Athens, Greece;2nd Department of Pathology, Attikon University Hospital, Athens University Medical School, Athens, Greece;4thDept of Internal Medicine, Attikon University Hospital, University of Athens Med. Sch. Athens, Greece;Department of Cytopathology, Alexandra Hospital, Athens, Greece;Department of Cytopathology, Attikon University Hospital, University of Athens Med. Sch. Athens, Greece;Department of Cytopathology, Attikon University Hospital, University of Athens Med. Sch. Athens, Greece;Department of Histology and Embryology, University of Athens Med. Sch. Athens, Greece;Department of Internal Medicine and Oncology, University of Athens Med. Sch. Athens, Greece;

关键词: Human Papilloma Virus;    Cervical Intraepithelial Neoplasia;    Positive Likelihood Ratio;    Human Papilloma Virus Infection;    Human Papilloma Virus Type;   

DOI  :  10.1186/1471-2407-10-53

 received in 2009-06-11, accepted in 2010-02-22,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundWe evaluated two molecular methods of HPV detection and their correlation with cytological and histological diagnosis in a large sample of Greek women.MethodsAll women with liquid-based cytology performed at a University Hospital between 2000 and 2003 were included. The Hybrid Capture 2 (HC2) kit and in house Polymerase Chain Reaction (PCR) were used for HPV DNA detection. Cervical biopsy was performed for women with ASCUS+ cytology, HPV detection, or abnormal colposcopy. Positive (PLR) and negative (NLR) likelihood ratios were calculated for cytology and HPV molecular testing for the prediction of CIN2 and greater histology.ResultsOf the 1270 women evaluated 241 (18.5%) had abnormal cytology. Cytology diagnosed high-grade squamous intraepithelial lesion (HSIL) or invasive carcinoma in 21(1.7%) cases whereas 26 (2%) women had CIN2+ or greater histology. PCR detected HPV in 397/1270 (31.3%) and HC2 in 260/1270 (20.4%) samples. Both molecular tests exhibited high reproducibility (Cohen's kappa value 0.691, 95% CI: 0.664 - 0.718). Positive likelihood ratios (PLR) of 9.4, 3.8 and 3.4 and negative likelihood ratios of 0.13, 0.21, and 0 were noted for ≥ LSIL, any positive HC2 or any positive PCR-HPV testing, for predicting CIN2+ histology, respectively. All CIN 3+ lesions harbored high risk oncogenic HPV type infections.ConclusionsHPV infection was found in a large proportion of this population and was associated with CIN 2/3 lesions and infiltrating carcinomas. Thin prep testing and HPV detection by HC2 or PCR performed very well with regards to identifying high grade lesions in an environment with experienced examiners.

【 授权许可】

Unknown   
© Tsiodras et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.