Membrane proteomic analysis of pancreatic cancer cells

Journal of Biomedical Science

Research

Vay Liang W Go¹  Xiaojun Liu²  Min Zhang²  Shen Hu³ 

[1] UCLA Center of Excellence in Pancreatic Diseases, 90095, Los Angeles, CA, USA;UCLA School of Dentistry & Dental Research Institute, 90095, Los Angeles, CA, USA;UCLA School of Dentistry & Dental Research Institute, 90095, Los Angeles, CA, USA;UCLA Jonsson Comprehensive Cancer Center, 90095, Los Angeles, CA, USA;

关键词: Pancreatic Cancer;    Pancreatic Cancer Cell;    Pancreatic Ductal Adenocarcinoma;    PDAC Cell;    Membrane Proteomes;   

DOI  :  10.1186/1423-0127-17-74

 received in 2010-05-05, accepted in 2010-09-13,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundPancreatic cancer is one of the most aggressive human tumors due to its high potential of local invasion and metastasis. The aim of this study was to characterize the membrane proteomes of pancreatic ductal adenocarcinoma (PDAC) cells of primary and metastatic origins, and to identify potential target proteins related to metastasis of pancreatic cancer.MethodsMembrane/membrane-associated proteins were isolated from AsPC-1 and BxPC-3 cells and identified with a proteomic approach based on SDS-PAGE, in-gel tryptic digestion and liquid chromatography with tandem mass spectrometry (LC-MS/MS). X! Tandem was used for database searching against the SwissProt human protein database.ResultsWe identified 221 & 208 proteins from AsPC-1 and BxPC-3 cells, respectively, most of which are membrane or membrane-associated proteins. A hundred and nine proteins were found in both cell lines while the others were present in either AsPC-1 or BxPC-3 cells. Differentially expressed proteins between two cell lines include modulators of cell adhesion, cell motility or tumor invasion as well as metabolic enzymes involved in glycolysis, tricarboxylic acid cycle, or nucleotide/lipid metabolism.ConclusionMembrane proteomes of AsPC-1 (metastatic) and BxPC-3 (primary) cells are remarkably different. The differentially expressed membrane proteins may serve as potential targets for diagnostic and therapeutic interventions.

【 授权许可】

Unknown   
© Liu et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.