Gene expression profiling in the stress control brain region hypothalamic paraventricular nucleus reveals a novel gene network including Amyloid beta Precursor Protein

BMC Genomics

Research Article

Dietrich Trümbach¹  Theo Rein²  Benno Pütz²  Jan M Deussing²  Rainer Landgraf²  Ludwig Czibere²  Markus Panhuysen²  Florian Holsboer²  Inge Sillaber²  Amalia Tsolakidou³  Wolfgang Wurst⁴ 

[1] Helmholtz Centre and Technical University Munich, Institute for Developmental Genetics, Neuherberg, Germany; and German Centre for Neurodegenerative Diseases, Munich, Germany;Max-Planck Institute of Psychiatry, Munich, Germany;Max-Planck Institute of Psychiatry, Munich, Germany;Department of Psychiatry and Psychotherapy, Technical University of Munich, Germany;Max-Planck Institute of Psychiatry, Munich, Germany;Helmholtz Centre and Technical University Munich, Institute for Developmental Genetics, Neuherberg, Germany; and German Centre for Neurodegenerative Diseases, Munich, Germany;

关键词: Forced Swimming;    Inbred Mouse Strain;    Signal Transduction Molecule;    Guanine Nucleotide Binding Protein;    Hypothalamic Paraventricular Nucleus;   

DOI  :  10.1186/1471-2164-11-546

 received in 2010-03-23, accepted in 2010-10-08,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundThe pivotal role of stress in the precipitation of psychiatric diseases such as depression is generally accepted. This study aims at the identification of genes that are directly or indirectly responding to stress. Inbred mouse strains that had been evidenced to differ in their stress response as well as in their response to antidepressant treatment were chosen for RNA profiling after stress exposure. Gene expression and regulation was determined by microarray analyses and further evaluated by bioinformatics tools including pathway and cluster analyses.ResultsForced swimming as acute stressor was applied to C57BL/6J and DBA/2J mice and resulted in sets of regulated genes in the paraventricular nucleus of the hypothalamus (PVN), 4 h or 8 h after stress. Although the expression changes between the mouse strains were quite different, they unfolded in phases over time in both strains. Our search for connections between the regulated genes resulted in potential novel signalling pathways in stress. In particular, Guanine nucleotide binding protein, alpha inhibiting 2 (GNAi2) and Amyloid β (A4) precursor protein (APP) were detected as stress-regulated genes, and together with other genes, seem to be integrated into stress-responsive pathways and gene networks in the PVN.ConclusionsThis search for stress-regulated genes in the PVN revealed its impact on interesting genes (GNAi2 and APP) and a novel gene network. In particular the expression of APP in the PVN that is governing stress hormone balance, is of great interest. The reported neuroprotective role of this molecule in the CNS supports the idea that a short acute stress can elicit positive adaptational effects in the brain.

【 授权许可】

Unknown   
© Tsolakidou et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.