Influence of 5 major Salmonella pathogenicity islands on NK cell depletion in mice infected with Salmonella enterica serovar Enteritidis

BMC Microbiology

Research Article

Hana Havlickova¹  Ivan Rychlik¹  Petra Ondrackova¹  Frantisek Sisak¹  Jiri Volf¹  Vladimir Kummer¹  Alena Sebkova¹  Martin Faldyna¹  Daniela Karasova¹ 

[1] Veterinary Research Institute, Hudcova 70, 621 00, Brno, Czech Republic;

关键词: Wild Type Strain;    Pathogenicity Island;    Splenic Lymphocyte;    Lymphocyte Proliferation Assay;    Salmonella Pathogenicity Island;   

DOI  :  10.1186/1471-2180-10-75

 received in 2009-07-29, accepted in 2010-03-12,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundIn this study we were interested in the colonisation and early immune response of Balb/C mice to infection with Salmonella Enteritidis and isogenic pathogenicity island free mutants.ResultsThe virulence of S. Enteritidis for Balb/C mice was exclusively dependent on intact SPI-2. Infections with any of the mutants harbouring SPI-2 (including the mutant in which we left only SPI-2 but removed SPI-1, SPI-3, SPI-4 and SPI-5) resulted in fatalities, liver injures and NK cell depletion from the spleen. The infection was of minimal influence on counts of splenic CD4 CD8 T lymphocytes and γδ T-lymphocytes although a reduced ability of splenic lymphocytes to respond to non-specific mitogens indicated general immunosuppression in mice infected with SPI-2 positive S. Enteritidis mutants. Further investigations showed that NK cells were depleted also in blood but not in the caecal lamina propria. However, NK cell depletion was not directly associated with the presence of SPI-2 and was rather an indicator of virulence or avirulence of a particular mutant because the depletion was not observed in mice infected with other attenuated mutants such as lon and rfaL.ConclusionsThe virulence of S. Enteritidis for Balb/C mice is exclusively dependent on the presence of SPI-2 in its genome, and a major hallmark of the infection in terms of early changes in lymphocyte populations is the depletion of NK cells in spleen and blood. The decrease of NK cells in circulation can be used as a marker of attenuation of S. Enteritidis mutants for Balb/C mice.

【 授权许可】

Unknown   
© Karasova et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.