Global transcriptome profiling of wild soybean (Glycine soja) roots under NaHCO3treatment

BMC Plant Biology

Research Article

Yong Li¹  Ying Ge¹  Yan-Ming Zhu¹  De-Kang Lv¹  Xi Bai¹  Wei Ji¹  Hua Cai¹  Dianjing Guo² 

[1] Plant Bioengineering Laboratory, The College of Life Sciences, Northeast Agricultural University, Harbin, China;State Key Lab for Agrobiotechnology and Department of Biology, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong;

关键词: Wild Soybean;    Alkaline Stress;    Alkali Stress;    Glycine Soja;    Intracellular Traffic;   

DOI  :  10.1186/1471-2229-10-153

 received in 2009-11-25, accepted in 2010-07-26,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundPlant roots are the primary site of perception and injury for saline-alkaline stress. The current knowledge of saline-alkaline stress transcriptome is mostly focused on saline (NaCl) stress and only limited information on alkaline (NaHCO3) stress is available.ResultsUsing Affymetrix® Soybean GeneChip®, we conducted transcriptional profiling on Glycine soja roots subjected to 50 mmol/L NaHCO3 treatment. In a total of 7088 probe sets, 3307 were up-regulated and 5720 were down-regulated at various time points. The number of significantly stress regulated genes increased dramatically after 3 h stress treatment and peaked at 6 h. GO enrichment test revealed that most of the differentially expressed genes were involved in signal transduction, energy, transcription, secondary metabolism, transporter, disease and defence response. We also detected 11 microRNAs regulated by NaHCO3 stress.ConclusionsThis is the first comprehensive wild soybean root transcriptome analysis under alkaline stress. These analyses have identified an inventory of genes with altered expression regulated by alkaline stress. The data extend the current understanding of wild soybean alkali stress response by providing a set of robustly selected, differentially expressed genes for further investigation.

【 授权许可】

Unknown   
© Ge et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.