Continuous wavelet transform for solving the problem of minor components in quantitation of pharmaceuticals: a case study on the mixture of ibuprofen and phenylephrine with its degradation products

BMC Chemistry

Research

Khadiga M. Kelani¹  Yasmin M. Fayez¹  Said A. Hassan¹  Reham A. Fekry² 

[1] Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr El-Aini Street, 11562, Cairo, Egypt;Analytical Chemistry Department, Faculty of Pharmacy, Modern University for Technology and Information, El-Hadaba El-Wosta, Mokatam, 5th district, Cairo, Egypt;

关键词: Derivative ratio-zero crossing;    Derivative spectrophotometry;    Ibuprofen;    Minor component;    Numerical differentiation;    Phenylephrine;    Wavelet transform;   

DOI  :  10.1186/s13065-023-01059-1

 received in 2023-06-04, accepted in 2023-10-16,  发布年份 2023

来源: Springer

【 摘 要 】

The presence of minor components represents a challenging problem in spectrophotometric analysis of pharmaceuticals. If one component has a low absorptivity or present in a low concentration compared to the other components, this will hinder its quantitation by spectrophotometric methods. Continuous Wavelet Transform (CWT) as a signal processing technique was utilized to figure out a solution to such a problem. A comparative study was established between traditional derivative spectrophotometry (Numerical Differentiation, ND) and CWT to indicate the advantages and limitations of each technique and possibility of solving the problem of minor components. A mixture of ibuprofen (IBU) and phenylephrine (PHE) with its degradation products forming a ternary mixture was used for comparing the two techniques. The two techniques were applied on raw spectral data and on ratio spectra data resulting in four methods, namely ND, CWT, Derivative Ratio-Zero Crossing (DRZC) and Continuous Wavelet Transform Ratio-Zero Crossing (CWTR-ZC) methods. By comparing the results in laboratory prepared mixtures, CWT technique showed advantages in analysis of mixtures with minor components than ND. The proposed methods were validated according to the ICH guideline Q2(R1), where their linearity was established with correlation coefficient ranging from 0.9995 to 0.9999. The linearity was in the range 3–40 μg/mL for PHE in all methods, while for IBU it was 20–180 and 30–180 μg/mL in CWT and ND methods, respectively. The CWT methods were applied for quantitative determination of the drugs in their dosage form showing the ability of the methods to quantitate minor components in pharmaceutical formulations.

【 授权许可】

CC BY   
© Springer Nature Switzerland AG 2023

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