Different effectiveness of closed embryo culture system with time-lapse imaging (EmbryoScopeTM) in comparison to standard manual embryology in good and poor prognosis patients: a prospectively randomized pilot study

Reproductive Biology and Endocrinology

Research

Yan-Guang Wu¹  Emanuela Lazzaroni-Tealdi¹  Lin Zhang¹  Qi Wang¹  Sarah K. Darmon¹  David F. Albertini²  Vitaly A. Kushnir³  David H. Barad⁴  Norbert Gleicher⁵ 

[1] The Center for Human Reproduction, 21 East 69th Street, 10021, New York, NY, USA;The Center for Human Reproduction, 21 East 69th Street, 10021, New York, NY, USA;Department of Molecular and Integrative Physiology, The University of Kansas School of Medicine, 64109, Wichita, KS, USA;The Center for Human Reproduction, 21 East 69th Street, 10021, New York, NY, USA;Department of Obstetrics and Gynecology, Wake Forest University, 27106, Winston Salem, NC, USA;The Center for Human Reproduction, 21 East 69th Street, 10021, New York, NY, USA;The Foundation for Reproductive Medicine, 10021, New York, NY, USA;Department of Obstetrics and Gynecology, Albert Einstein College of Medicine, 10461, Bronx, NY, USA;The Center for Human Reproduction, 21 East 69th Street, 10021, New York, NY, USA;The Foundation for Reproductive Medicine, 10021, New York, NY, USA;Stem Cell Biology and Molecular Embryology Laboratory, The Rockefeller University, 10065, New York, NY, USA;

关键词: In vitro fertilization (IVF);    Time laps photography;    Closed incubation systems;    Embryology;    Embryo quality;    Cost-effectiveness;   

DOI  :  10.1186/s12958-016-0181-x

 received in 2016-05-24, accepted in 2016-08-04,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundPreviously manual human embryology in many in vitro fertilization (IVF) centers is rapidly being replaced by closed embryo incubation systems with time-lapse imaging. Whether such systems perform comparably to manual embryology in different IVF patient populations has, however, never before been investigated.We, therefore, prospectively compared embryo quality following closed system culture with time-lapse photography (EmbryoScope™) and standard embryology.We performed a two-part prospectively randomized study in IVF (clinical trial # NCT92256309). Part A involved 31 infertile poor prognosis patients prospectively randomized to EmbryoScope™ and standard embryology. Part B involved embryos from 17 egg donor-recipient cycles resulting in large egg/embryo numbers, thus permitting prospectively alternative embryo assignments to EmbryoScope™ and standard embryology.We then compared pregnancy rates and embryo quality on day-3 after fertilization and embryologist time utilized per processed embryo.ResultsPart A revealed in poor prognosis patients no differences in day-3 embryo scores, implantation and clinical pregnancy rates between EmbryoScope™ and standard embryology. The EmbryoScope™, however, more than doubled embryology staff time (P < 0.0001). In Part B, embryos grown in the EmbyoScope™ demonstrated significantly poorer day-3 quality (depending on embryo parameter between P = 0.005 and P = 0.01). Suspicion that conical culture dishes of the EmbryoScope™ (EmbryoSlide™) may be the cause was disproven when standard culture dishes demonstrated no outcome difference in standard incubation.ConclusionsThough due to small patient numbers preliminary, this study raises concerns about the mostly uncontrolled introduction of closed incubation systems with time lapse imaging into routine clinical embryology. Appropriately designed and powered prospectively randomized studies appear urgently needed in well-defined patient populations before the uncontrolled utilization of these instruments further expands.Trial registrationNCT02246309 Registered September 18, 2014.

【 授权许可】

CC BY   
© The Author(s). 2016