Serotonin-mediated modulation of Na+/K+ pump current in rat hippocampal CA1 pyramidal neurons

BMC Neuroscience

Research Article

Su Wen Su¹  Fang Guo¹  Wen Ya Li¹  Xiao Lu Shi¹  Xu Liu¹  Yong Li Wang¹  Li Nan Zhang²  Hui Cai Guo³ 

[1] Department of Pharmacology, Hebei Medical University, 361 Zhongshan East Road, 050017, Shijiazhuang, China;Department of Pharmacology, Hebei Medical University, 361 Zhongshan East Road, 050017, Shijiazhuang, China;Department of Pharmacy, College of Chemical and Pharmaceutical Engineering, Hebei University of Science and Technology, 70 Yuhua East Road, 050018, Shijiazhuang, China;Department of Toxicology, Hebei Medical University, 361 Zhongshan East Road, 050017, Shijiazhuang, China;

关键词: Pyramidal Neuron;    Hippocampal Slice;    Pump Activity;    Pump Current;    Hippocampal Dependent Task;   

DOI  :  10.1186/1471-2202-13-10

 received in 2010-03-14, accepted in 2012-01-19,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundThe aim of this study was to investigate whether serotonin (5-hydroxytryptamine, 5-HT) can modulate Na+/K+ pump in rat hippocampal CA1 pyramidal neurons.Results5-HT (0.1, 1 mM) showed Na+/K+ pump current (Ip) densities of 0.40 ± 0.04, 0.34 ± 0.03 pA/pF contrast to 0.63 ± 0.04 pA/pF of the control of 0.5 mM strophanthidin (Str), demonstrating 5-HT-induced inhibition of Ip in a dose-dependent manner in hippocampal CA1 pyramidal neurons. The effect was partly attenuated by ondasetron, a 5-HT3 receptor (5-HT3R) antagonist, not by WAY100635, a 5-HT1AR antagonist, while 1-(3-Chlorophenyl) biguanide hydrochloride (m-CPBG), a 5-HT3R specific agonist, mimicked the effect of 5-HT on Ip.Conclusion5-HT inhibits neuronal Na+/K+ pump activity via 5-HT3R in rat hippocampal CA1 pyramidal neurons. This discloses novel mechanisms for the function of 5-HT in learning and memory, which may be a useful target to benefit these patients with cognitive disorder.

【 授权许可】

Unknown   
© Zhang et al; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.