Prevalence of nasal colonisation by methicillin-sensitive and methicillin-resistant Staphylococcus aureus among healthcare workers and students in Madagascar

BMC Infectious Diseases

Research Article

Raphael Rakotozandrindrainy¹  Tsiriniaina Razafindrabe¹  Jean Noel Heriniaina¹  Jean Philibert Rakotondrainiarivelo¹  Ralf Matthias Hagen²  Volker Micheel²  Hagen Frickmann³  Sabine Crusius⁴  Benedikt Hogan⁵  Hassan Al-Emran⁵  Norbert Georg Schwarz⁵  Jürgen May⁵  Andreas Hahn⁵  Anna Jaeger⁵  Denise Dekker⁵  Sven Poppert⁶ 

[1] Department of Microbiology and Parasitology, University of Antananarivo, B.P. 175, Antananarivo, Madagascar;Department of Tropical Medicine at the Bernhard Nocht Institute, German Armed Forces Hospital of Hamburg, Bernhard-Nocht-Str. 74, D-20359, Hamburg, Germany;Department of Tropical Medicine at the Bernhard Nocht Institute, German Armed Forces Hospital of Hamburg, Bernhard-Nocht-Str. 74, D-20359, Hamburg, Germany;Institute for Medical Microbiology, Virology and Hygiene, University Hospital of Rostock, Schillingallee 70, D-18057, Rostock, Germany;Institute for Medical Microbiology, Virology and Hygiene, University Hospital of Rostock, Schillingallee 70, D-18057, Rostock, Germany;Research Group Infectious Disease Epidemiology, Bernhard Nocht Institute for Tropical Medicine (BNITM), Bernhard-Nocht-Str. 74, D-20359, Hamburg, Germany;University Medical Center, Hamburg-Eppendorf, Martinistr. 52, 20251, Hamburg, Germany;

关键词: Healthcare Worker;    Mupirocin;    Nasal Swab;    Nasal Carriage;    Nasal Carriage Rate;   

DOI  :  10.1186/s12879-016-1733-6

 received in 2016-03-31, accepted in 2016-07-22,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundMethicillin-resistant Staphylococcus aureus (MRSA) clones pose a significant threat to hospitalised patients because the bacteria can be transmitted by asymptomatic carriers within healthcare facilities. To date, nothing is known about the prevalence of S. aureus and MRSA among healthcare workers in Madagascar.The objective of our study was to examine the prevalence and clonal epidemiology of nasal S. aureus and MRSA among healthcare workers and non-medical University students in Antananarivo, Madagascar.MethodsThis cross sectional study screened nasal swabs taken from students and healthcare workers for S. aureus. Multiplex PCR was performed to identify S. aureus-specific (nuc), MRSA-specific mecA and mecC genes, Panton-Valentine leukocidin (PVL) (lukF-PV), and toxic shock syndrome toxin-1 (TSST-1) specific genes in methicillin-sensitive S. aureus (MSSA) and MRSA isolates. Staphylococcus protein A gene (spa) typing was performed for all confirmed MRSA isolates. The frequency distribution of nasal S. aureus and MRSA of healthcare workers and non-medical students was compared using Pearson’s χ2 test.ResultsOf 1548 nasal swabs tested, 171 (11 %) were positive for S. aureus; 20 (1.3 %) of these isolates were identified as MRSA. S. aureus was detected in 91 of 863 healthcare workers (10.4 %) and in 80 (11.8 %) of 685 students; however, 14 (1.5 %) healthcare workers carried MRSA compared with six (0.9 %) students. Nasal carriage of S. aureus and MRSA was more prevalent in women than in men, and 21 (11.7 %) S. aureus isolates were PVL-positive and 36 (21 %) were TSST-1 positive. The mecC gene was not detected in any isolates. Five different spa types were identified, with spa type t186 being the predominant MRSA clone (16/20).ConclusionThe results of the present study reveal a low frequency of S. aureus and MRSA nasal carriage in both students and healthcare workers from Antananarivo, Madagascar. The predominant MRSA clone (t186) was previously described in hospitalised patients in Madagascar.

【 授权许可】

CC BY   
© The Author(s). 2016