Methods for genetic transformation of filamentous fungi

Microbial Cell Factories

Review

Weiwen Cai¹  Dandan Li¹  Jun Lin²  Yu Tang³ 

[1] Institute of Apply Genomics, Fuzhou University, No.2 Xueyuan Road, 350108, Fuzhou, China;College of Biological Science and Engineering, Fuzhou University, No.2 Xueyuan Road, 350108, Fuzhou, China;Institute of Apply Genomics, Fuzhou University, No.2 Xueyuan Road, 350108, Fuzhou, China;School of Basic Medical Sciences, Fujian Medical University, No.1 Xuefubei Road, 350122, Fuzhou, China;College of Biological Science and Engineering, Fuzhou University, No.2 Xueyuan Road, 350108, Fuzhou, China;Triplex International Biosciences (China) Co. LTD, 361100, Xiamen, China;

关键词: Filamentous fungi;    Protoplast-mediated transformation;    Agrobacterium;    Electroporation;    Biolistic method;    Shock-wave-mediated transformation;   

DOI  :  10.1186/s12934-017-0785-7

 received in 2017-05-11, accepted in 2017-09-26,  发布年份 2017

来源: Springer

【 摘 要 】

Filamentous fungi have been of great interest because of their excellent ability as cell factories to manufacture useful products for human beings. The development of genetic transformation techniques is a precondition that enables scientists to target and modify genes efficiently and may reveal the function of target genes. The method to deliver foreign nucleic acid into cells is the sticking point for fungal genome modification. Up to date, there are some general methods of genetic transformation for fungi, including protoplast-mediated transformation, Agrobacterium-mediated transformation, electroporation, biolistic method and shock-wave-mediated transformation. This article reviews basic protocols and principles of these transformation methods, as well as their advantages and disadvantages.

【 授权许可】

CC BY   
© The Author(s) 2017