Comprehensive Cardiovascular magnetic resonance of myocardial mechanics in mice using three-dimensional cine DENSE

Journal of Cardiovascular Magnetic Resonance

Technical Notes

Brent A French¹  Xiaodong Zhong²  Bruce S Spottiswoode³  Andrew D Gilliam⁴  Lauren B Gibberman⁵  Craig H Meyer⁶  Frederick H Epstein⁶ 

[1] Biomedical Engineering Department, University of Virginia, Charlottesville, USA;MR R&D Collaborations, Siemens Healthcare, Atlanta, USA;MRC/UCT Medical Imaging Research Unit, University of Cape Town, Cape Town, South Africa;R&D Department, A.D. Gilliam Consulting, Providence, USA;Radiology Department, University of Virginia, Charlottesville, USA;Radiology Department, University of Virginia, Charlottesville, USA;Biomedical Engineering Department, University of Virginia, Charlottesville, USA;

关键词: Cardiovascular Magnetic Resonance;    Mouse Heart;    Cardiac Phase;    Myocardial Mechanic;    Partition Direction;   

DOI  :  10.1186/1532-429X-13-83

 received in 2011-07-06, accepted in 2011-12-30,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundQuantitative noninvasive imaging of myocardial mechanics in mice enables studies of the roles of individual genes in cardiac function. We sought to develop comprehensive three-dimensional methods for imaging myocardial mechanics in mice.MethodsA 3D cine DENSE pulse sequence was implemented on a 7T small-bore scanner. The sequence used three-point phase cycling for artifact suppression and a stack-of-spirals k-space trajectory for efficient data acquisition. A semi-automatic 2D method was adapted for 3D image segmentation, and automated 3D methods to calculate strain, twist, and torsion were employed. A scan protocol that covered the majority of the left ventricle in a scan time of less than 25 minutes was developed, and seven healthy C57Bl/6 mice were studied.ResultsUsing these methods, multiphase normal and shear strains were measured, as were myocardial twist and torsion. Peak end-systolic values for the normal strains at the mid-ventricular level were 0.29 ± 0.17, -0.13 ± 0.03, and -0.18 ± 0.14 for Err , Ecc , and Ell , respectively. Peak end-systolic values for the shear strains were 0.00 ± 0.08, 0.04 ± 0.12, and 0.03 ± 0.07 for Erc , Erl , and Ecl , respectively. The peak end-systolic normalized torsion was 5.6 ± 0.9°.ConclusionsUsing a 3D cine DENSE sequence tailored for cardiac imaging in mice at 7 T, a comprehensive assessment of 3D myocardial mechanics can be achieved with a scan time of less than 25 minutes and an image analysis time of approximately 1 hour.

【 授权许可】

Unknown   
© Zhong et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.