Mycobacterium tuberculosis causing tuberculous lymphadenitis in Maputo, Mozambique

BMC Microbiology

Research Article

Gunilla Källenius¹  Tuija Koivula²  Margarida Correia-Neves³  Ramona Groenheit⁴  Solomon Ghebremichael⁴  Adelina Machado⁵  Leguesse Massawo⁶  José Maiane Matavele⁶  Sofia Omar Viegas⁷  Matos Alberto⁸  Eliane Monteiro⁸  Fabíola Couto Fernandes⁹  Carla Carrilho⁹  Nalin Rastogi^1,10  David Couvin^1,10 

[1] Department of Clinical Science and Education, Karolinska Institutet, Stockholm, Sweden;Department of Clinical Science and Education, Karolinska Institutet, Stockholm, Sweden;Department of Microbiology, Public Health Agency of Sweden, Solna, Sweden;Department of Clinical Science and Education, Karolinska Institutet, Stockholm, Sweden;Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Braga, Portugal;ICVS/3B’s, PT Government Associate Laboratory, Braga/Guimarães, Portugal;Department of Microbiology, Public Health Agency of Sweden, Solna, Sweden;Faculty of Veterinary, Eduardo Mondlane University, Maputo, Mozambique;National Institute of Health, Ministry of Health, Maputo, Mozambique;National Institute of Health, Ministry of Health, Maputo, Mozambique;Faculty of Veterinary, Eduardo Mondlane University, Maputo, Mozambique;Department of Clinical Science and Education, Karolinska Institutet, Stockholm, Sweden;Pathology Service, Maputo Central Hospital, Ministry of Health, Maputo, Mozambique;Pathology Service, Maputo Central Hospital, Ministry of Health, Maputo, Mozambique;Department of Pathology, Faculty of Medicine, Eduardo Mondlane University, Maputo, Mozambique;WHO Supranational TB Reference Laboratory, Tuberculosis & Mycobacteria Unit, Institut Pasteur de la Guadeloupe, Abymes, Guadeloupe, France;

关键词: Human Immunodeficiency Virus;    Human Immunodeficiency Virus Infection;    Minimum Span Tree;    Human Immunodeficiency Virus Test;    Human Immunodeficiency Virus Status;   

DOI  :  10.1186/s12866-015-0603-5

 received in 2015-06-23, accepted in 2015-11-12,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundThe zoonosis bovine tuberculosis (TB) is known to be responsible for a considerable proportion of extrapulmonary TB. In Mozambique, bovine TB is a recognised problem in cattle, but little has been done to evaluate how Mycobacterium bovis has contributed to human TB. We here explore the public health risk for bovine TB in Maputo, by characterizing the isolates from tuberculous lymphadenitis (TBLN) cases, a common manifestation of bovine TB in humans, in the Pathology Service of Maputo Central Hospital, in Mozambique, during one year.ResultsAmong 110 patients suspected of having TBLN, 49 had a positive culture result. Of those, 48 (98 %) were positive for Mycobacterium tuberculosis complex and one for nontuberculous mycobacteria. Of the 45 isolates analysed by spoligotyping and Mycobacterial Interspersed Repetitive Unit – Variable Number Tandem Repeat (MIRU-VNTR), all were M. tuberculosis. No M. bovis was found.Cervical TBLN, corresponding to 39 (86.7 %) cases, was the main cause of TBLN and 66.7 % of those where from HIV positive patients.We found that TBLN in Maputo was caused by a variety of M. tuberculosis strains. The most prevalent lineage was the EAI (n = 19; 43.2 %). Particular common spoligotypes were SIT 48 (EAI1_SOM sublineage), SIT 42 (LAM 9), SIT 1 (Beijing) and SIT53 (T1), similar to findings among pulmonary cases.ConclusionsM. tuberculosis was the main etiological agent of TBLN in Maputo. M. tuberculosis genotypes were similar to the ones causing pulmonary TB, suggesting that in Maputo, cases of TBLN arise from the same source as pulmonary TB, rather than from an external zoonotic source. Further research is needed on other forms of extrapulmonary TB and in rural areas where there is high prevalence of bovine TB in cattle, to evaluate the risk of transmission of M. bovis from cattle to humans.

【 授权许可】

CC BY   
© Viegas et al. 2015