STIL, a peculiar molecule from styles, specifically dephosphorylates the pollen receptor kinase LePRK2 and stimulates pollen tube growth in vitro

BMC Plant Biology

Research Article

Tamara M Salem¹  María A Mazzella¹  Diego L Wengier¹  Jorge P Muschietti²  Sheila McCormick³ 

[1] Instituto de Ingeniería Genética y Biología Molecular (INGEBI), CONICET, Vuelta de Obligado 2490, 1428, Buenos Aires, Argentina;Instituto de Ingeniería Genética y Biología Molecular (INGEBI), CONICET, Vuelta de Obligado 2490, 1428, Buenos Aires, Argentina;Departamento de Fisiología y Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos, Aires, Argentina;Plant Gene Expression Center, United States Department of Agriculture/Agricultural Research Service, 800 Buchanan Street, 94710, Albany, California, USA;Department of Plant and Microbial Biology, University of California at Berkeley, 94720, Berkeley, California, USA;

关键词: Pollen Tube;    Pollen Tube Growth;    Pollen Tube Length;    Pollen Tube Growth Rate;    Amino Acid Determination;   

DOI  :  10.1186/1471-2229-10-33

 received in 2009-08-11, accepted in 2010-02-22,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundLePRK1 and LePRK2 are two pollen receptor kinases localized to the plasma membrane, where they are present in a high molecular weight complex (LePRK complex). LePRK2 is phosphorylated in mature and germinated pollen, but is dephosphorylated when pollen membranes are incubated with tomato or tobacco style extracts.ResultsHere we show that LePRK2 dephosphorylation is mediated by a heat-, acid-, base-, DTT- and protease-resistant component from tobacco styles. Using LePRK2 phosphorylation as a tracking assay for purification, style exudates were subjected to chloroform extraction, anionic exchange, and C18 reverse-phase chromatography columns. We finally obtained a single ~3,550 Da compound (as determined by UV-MALDI-TOF MS) that we named STIL (for Style Interactor for LePRKs). STIL increased pollen tube lengths of in vitro germinated pollen in a dose-dependent manner.ConclusionWe propose that the LePRK complex perceives STIL, resulting in LePRK2 dephosphorylation and an increase in pollen tube growth.

【 授权许可】

Unknown   
© Wengier et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.