High prevalence of CXCR4 usage among treatment-naive CRF01_AE and CRF51_01B-infected HIV-1 subjects in Singapore

BMC Infectious Diseases

Research Article

Li Lin¹  Mei Ting Tan²  Wei Xin Khong²  Arlene Chua²  Kah Ying Ng²  Joe Yap Kwan²  Yee Sin Leo²  Palvinder Kaur²  Oon Tek Ng²  Kuan Kiat Chew²  Thomas C Quinn³  Oliver Laeyendecker³ 

[1] Department of Infectious Disease, Jurong General Hospital, 159964, Singapore, Singapore;Institute of Infectious Disease and Epidemiology, Communicable Disease Centre, Tan Tock Seng Hospital, 308433, Singapore, Singapore;Johns Hopkins School of Medicine, Baltimore, MD, USA;Laboratory of Immunoregulation, Division of Intramural Research, National Institute of Allergy and Infectious Disease, National Institutes of Health, Baltimore, MD, USA;

关键词: CXCR4 usage;    HIV-1;    treatment-naïve;   

DOI  :  10.1186/1471-2334-13-90

 received in 2012-10-31, accepted in 2013-02-08,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundRecent studies suggest HIV-1 inter-subtype differences in co-receptor usage. We examined the correlation between HIV-1 subtype and co-receptor usage among treatment-naïve HIV-1 subjects in Singapore. Additionally, we investigated whether the subtype co-receptor association was influenced by stage of infection.MethodsV3 sequences of HIV-1 envelope protein gp120 were obtained from 110 HIV treatment-naïve patients and genotypic co-receptor tropism determination was performed using Geno2pheno. Two false-positive rate (FPR) cut-offs, 10% and 5.75% were selected for tropism testing.ResultsSubtype assignment of viral strains from 110 HIV-infected individuals based on partial sequencing of HIV-1 pol, gp120 and gp41 were as follows: 27 subtype B, 64 CRF01_AE, 10 CRF51_01B, and 9 other subtypes. At FPR=10%, 10 (100%) CRF51_01B-infected subjects and 26 (40.6%) CRF01_AE-infected subjects had CXCR4-using virus, compared to 7 (25.9%) subtype B subjects and 1 (11.1%) CRF33_01B-infected subject (P < 0.001). At FPR=5.75%, 10 (100%) CRF51_01B-infected subjects and 20 (31.3%) CRF01_AE-infected subjects had CXCR4-using virus, compared to 4 (14.8%) subtype B and 1 (11.1%) CRF33_01B-infected subjects (P < 0.001). Among those with evidence of seroconversion within 2 years prior to study enrolment, 100% of CRF51_01B-infected subjects had CXCR4-using virus, independent of Geno2pheno FPR.ConclusionCRF51_01B and CRF01_AE-infected individuals have higher prevalence of CXCR4-usage compared to subtype B infected individuals. Further studies examining these differences could help optimise the use of CCR5-antagonist in populations with these subtypes, and increase our understanding of HIV-1 biology.

【 授权许可】

CC BY   
© Ng et al.; licensee BioMed Central Ltd. 2013