Next generation sequencing and de novo transcriptome analysis of Costus pictus D. Don, a non-model plant with potent anti-diabetic properties

BMC Genomics

Research Article

Ramasamy S Annadurai¹  S Murugesan²  Raja C Mugasimangalam³  Nandita Mullapudi³  Sreeja Gopinathan³  Mohan AVSK Katta³  Sudha Narayana Rao³  Sunjay Jude Fernandes³  Vasanthan Jayakumar³  Santosh Prasad Sarma³  Sanchita Anand³ 

[1] Currently at MTP Biology, ITC R&D Centre, Peenya Industrial Area, 1st Phase, 560 058, Bangalore, Karnataka, India;Division of Bioprospecting, Institute of Forest Genetics and Tree Breeding, R.S.Puram, 641 002, Coimbatore, Tamilnadu, India;Research and Development Unit, Genotypic Technology Private Limited, Balaji Complex, Poojari Layout, 80 Feet Road, RMV 2nd Stage, 560094, Bangalore, Karnataka, India;

关键词: RNA-Seq;    Next Generation Sequencing (NGS);    de novo;    Abscisic Acid (ABA);    Costus pictus;    Diabetes mellitus;    Bixin;    Molecular signature;    PPAR agonist;    High Performance Liquid Chromatography (HPLC);   

DOI  :  10.1186/1471-2164-13-663

 received in 2012-06-29, accepted in 2012-11-08,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundPhyto-remedies for diabetic control are popular among patients with Type II Diabetes mellitus (DM), in addition to other diabetic control measures. A number of plant species are known to possess diabetic control properties. Costus pictus D. Don is popularly known as “Insulin Plant” in Southern India whose leaves have been reported to increase insulin pools in blood plasma. Next Generation Sequencing is employed as a powerful tool for identifying molecular signatures in the transcriptome related to physiological functions of plant tissues. We sequenced the leaf transcriptome of C. pictus using Illumina reversible dye terminator sequencing technology and used combination of bioinformatics tools for identifying transcripts related to anti-diabetic properties of C. pictus.ResultsA total of 55,006 transcripts were identified, of which 69.15% transcripts could be annotated. We identified transcripts related to pathways of bixin biosynthesis and geraniol and geranial biosynthesis as major transcripts from the class of isoprenoid secondary metabolites and validated the presence of putative norbixin methyltransferase, a precursor of Bixin. The transcripts encoding these terpenoids are known to be Peroxisome Proliferator-Activated Receptor (PPAR) agonists and anti-glycation agents. Sequential extraction and High Performance Liquid Chromatography (HPLC) confirmed the presence of bixin in C. pictus methanolic extracts. Another significant transcript identified in relation to anti-diabetic, anti-obesity and immuno-modulation is of Abscisic Acid biosynthetic pathway. We also report many other transcripts for the biosynthesis of antitumor, anti-oxidant and antimicrobial metabolites of C. pictus leaves.ConclusionSolid molecular signatures (transcripts related to bixin, abscisic acid, and geranial and geraniol biosynthesis) for the anti-diabetic properties of C. pictus leaves and vital clues related to the other phytochemical functions like antitumor, anti-oxidant, immuno-modulatory, anti-microbial and anti-malarial properties through the secondary metabolite pathway annotations are reported. The data provided will be of immense help to researchers working in the treatment of DM using herbal therapies.

【 授权许可】

Unknown   
© Annadurai et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.