Development of O-antigen gene cluster-specific PCRs for rapid typing six epidemic serogroups of Leptospira in China

BMC Microbiology

Research Article

Xiao-Fang Xin¹  Yi Zhong²  Guo-Ping Zhao³  Cheng-Song Cai⁴  Ping He⁴  Yong-Zhang Zhu⁴  Jin-Hong Qin⁴  Xiao-Li Lou⁴  Xiao-Kui Guo⁴  Xiu-Gao Jiang⁵  Sheng-Yue Wang⁶ 

[1] 1st Bacterial Vaccine Division, National Institute for the Control of Pharmaceutical and Biological Products, 100050, Beijing, China;Cell Biology/Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 200032, Shanghai, China;Key Laboratory of Systems Biology, Institute of Biochemistry, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 200032, Shanghai, China;Cell Biology/Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 200032, Shanghai, China;Key Laboratory of Systems Biology, Institute of Biochemistry, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 200032, Shanghai, China;Shanghai-MOST Key Laboratory of Health and Disease Genomics, Chinese National Human Genome Center at Shanghai, Shanghai, China;Department of Medical Microbiology and Parasitology, Institutes of Medical Sciences, Shanghai Jiao Tong University School of Medicine, 200025, Shanghai, China;National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention (ICDC, China CDC), PO Box 5, 102206, Changping, Beijing, China;Shanghai-MOST Key Laboratory of Health and Disease Genomics, Chinese National Human Genome Center at Shanghai, Shanghai, China;

关键词: Reference Strain;    Leptospirosis;    Microscopic Agglutination Test;    Glycosyltransferase Gene;    Draft Data;   

DOI  :  10.1186/1471-2180-10-67

 received in 2009-09-23, accepted in 2010-03-03,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundLeptospira is the causative agent of leptospirosis. The O-antigen is the distal part of the lipopolysaccharide, which is a key component of outer membrane of Gram-negative bacteria and confers serological specificity. The epidemiology and clinical characteristics of leptospirosis are relative to the serology based taxonomic unit. Identification of Leptospira strains by serotyping is laborious and has several drawbacks.ResultsIn this study, the O-antigen gene clusters of four epidemic Leptospira serogroups (serogroup Canicola, Autumnalis, Grippotyphosa and Hebdomadis) in China were sequenced and all genes were predicted in silico. Adding published sequences of two serogroups, Icterohaemorrhagiae (strain Lai and Fiocruz L1-130) and Sejroe (strain JB197 and L550), we identified six O-antigen-specific genes for six epidemic serogroups in China. PCR assays using these genes were developed and tested on 75 reference strains and 40 clinical isolates.ConclusionThe results show that the PCR-based assays can be reliable and alternative means for rapid typing of these six serogroups of Leptospira.

【 授权许可】

Unknown   
© Cai et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.