GLUT4, GLUT1, and GLUT8 are the dominant GLUT transcripts expressed in the murine left ventricle

Cardiovascular Diabetology

Original Investigation

Amanda Koenig¹  Lauren Aerni-Flessner¹  Maria Payne¹  Melissa Abi-Jaoude¹  Paul W Hruz² 

[1] Department of Pediatrics, Washington University School of Medicine, St. Louis, MO, USA;Department of Pediatrics, Washington University School of Medicine, St. Louis, MO, USA;Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, MO, USA;

关键词: Cardiomyopathy;    Diabetes;    Gene expression;    Glucose transport;    Heart failure;   

DOI  :  10.1186/1475-2840-11-63

 received in 2012-02-27, accepted in 2012-06-08,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundThe heart derives energy from a wide variety of substrates including fatty acids, carbohydrates, ketones, and amino acids. The healthy heart generates up to 30% of its ATP from glucose. Under conditions of cardiac injury or stress, the heart relies even more heavily on glucose as a source of fuel. Glucose is transported into the heart by members of the family of facilitative glucose transporters (GLUTs). While research examining the transport of glucose into the heart has primarily focused on the roles of the classical glucose transporters GLUT1 and GLUT4, little is known about the functions of more newly identified GLUT isoforms in the myocardium.MethodsIn this study the presence and relative RNA message abundance of each of the known GLUT isoforms was determined in left ventricular tissue from two commonly used inbred laboratory mouse strains (C57BL/6J and FVB/NJ) by quantitative real time PCR. Relative message abundance was also determined in GLUT4 null mice and in murine models of dilated and hypertrophic cardiomyopathy.ResultsGLUT4, GLUT1, and GLUT8 were found to be the most abundant GLUT transcripts in the normal heart, while GLUT3, GLUT10, and GLUT12 are present at relatively lower levels. Assessment of relative GLUT expression in left ventricular myocardium from mice with dilated cardiomyopathy revealed increased expression of GLUT1 with reduced levels of GLUT4, GLUT8, and GLUT12. Compensatory increase in the expression of GLUT12 was observed in genetically altered mice lacking GLUT4.ConclusionsGlucose transporter expression varies significantly among murine models of cardiac dysfunction and involves several of the class III GLUT isoforms. Understanding how these more newly identified GLUT isoforms contribute to regulating myocardial glucose transport will enhance our comprehension of the normal physiology and pathophysiology of the heart.

【 授权许可】

Unknown   
© Aerni-Flessner et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.