Resistance profiles, virulence and antimicrobial resistance genes of XDR S. Enteritidis and S. Typhimurium

AMB Express

Original Article

Ghada A. Badawy¹  Abdelazeem M. Algammal²  Reham M. El-Tarabili²  Tahani M. Alqahtani³  Enas A. Almanzalawi³  Amenah S. Al-otaibi⁴  Hanaa Ghabban⁴  Helal F. Hetta⁵  Wafaa A. Abd El-Ghany⁶  Nayera M. Alatfeehy⁷  Naira M. Abosleima⁷ 

[1]Botany Department, Faculty of Science, Fayoum University, 63514, Fayoum, Egypt

[2]Department of Biology, Faculty of Science, University of Tabuk, 46429, Umluj, Saudi Arabia

[3]Department of Bacteriology, Immunology, and Mycology, Faculty of Veterinary Medicine, Suez Canal University, 41522, Ismailia, Egypt

[4]Department of Biological Sciences, Faculty of Sciences, King Abdulaziz University, 21589, Jeddah, Saudi Arabia

[5]Department of Biology, Faculty of Science, University of Tabuk, 71491, Tabuk, Saudi Arabia

[6]Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, 71515, Assiut, Egypt

[7]Poultry Diseases Department, Faculty of Veterinary Medicine, Cairo University, 12211, Giza, Egypt

[8]Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Agriculture Research Center, 1261, Dokki, Giza, Egypt

关键词: S;    S.;    XDR;    Diseased broilers;    Virulence genes;    Antibiotic resistance genes;   

DOI  :  10.1186/s13568-023-01615-x

 received in 2023-07-29, accepted in 2023-09-22,  发布年份 2023

来源: Springer

【 摘 要 】

Avian salmonellosis is concomitant with high financial crises in the poultry industry as well as food-borne illness in man. The present study is designed to investigate the emergence of Salmonella Enteritidis and Salmonella Typhimurium in diseased broilers, resistance profiles, and monitoring virulence and antibiotic resistance genes. Consequently, 450 samples (cloacal swabs, liver, and spleen) were collected from 150 diseased birds from different farms in Giza Governorate, Egypt. Subsequently, the bacteriological examination was done. Afterward, the obtained Salmonella isolates were tested for serogrouping, antibiogram, PCR monitoring of virulence (invA, stn,hilA, and pefA), and antimicrobial resistance genes (blaTEM, blaCTX-M, blaNDM, ermA, sul1, tetA, and aadA1). The total prevalence of Salmonella in the examined diseased broilers was 9.3%, and the highest prevalence was noticed in cloacal swabs. Among the recovered Salmonella isolates (n = 35), 20 serovars were recognized as S. Enteritidis and 15 serovars were identified as S. Typhimurium. Almost 60% of the retrieved S. Enteritidis serovars were extensively drug-resistant (XDR) to seven antimicrobial classes and inherited sul1, blaTEM, tetA,blaCTX-M, ereA, and aadA1 genes. Likewise, 25% of the recovered S. Enteritidis serovars were multidrug-resistant (MDR) to six classes and have sul1, blaTEM, tetA,blaCTX-M, and ereA resistance genes. Also, 66.7% of the retrieved S. Typhimurium serovars were XDR to seven classes and have sul1, blaTEM, tetA,blaCTX-M, ereA, and aadA1 genes. Succinctly, this report underlined the reemergence of XDR S. Typhimurium and S. Enteritidis in broiler chickens. Meropenem and norfloxacin exposed a hopeful antimicrobial activity toward the re-emerging XDR S. Typhimurium and S. Enteritidis in broilers. Moreover, the recurrence of these XDR Salmonella strains poses a potential public health threat.

【 授权许可】

CC BY   
© Springer-Verlag GmbH Germany, part of Springer Nature 2023

【 图 表 】

Fig. 4

Fig. 1

Fig. 6

Fig. 2

12936_2017_1885_Article_IEq1.gif

Fig. 5