Phylogenetic and recombination analysis of human bocavirus 2

BMC Infectious Diseases

Research Article

Ming Zhang¹  Yu Jin²  Jinan Chen³  Ziqian Xu⁴  Canping Huang⁴  Huiying Li⁴  Miao Jin⁴  Jiemei Yu⁴  Zhao-jun Duan⁴  Weixia Cheng⁵ 

[1] Dept. of Epidemiology and Biostatistics and Faculty of Infectious Diseases, University of Georgia, 30602, Athens, GA, USA;Medical School of Nanjing University, Nanjing Children's Hospital, 210093, Nanjing, PR, China;School of Basic Medical Sciences, Lanzhou University, 730000, Lanzhou, PR, China;National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 100101, Beijing, PR, China;State Key Laboratory for Molecular Virology and Genetic Engineering, National Institute for Viral Disease Control and Prevention, China CDC, 100052, Beijing, PR, China;State Key Laboratory for Molecular Virology and Genetic Engineering, National Institute for Viral Disease Control and Prevention, China CDC, 100052, Beijing, PR, China;Medical School of Nanjing University, Nanjing Children's Hospital, 210093, Nanjing, PR, China;

关键词: Gastroenteritis;    Stool Sample;    Bocavirus;    Human Bocavirus;    RDP3 Package;   

DOI  :  10.1186/1471-2334-11-50

 received in 2010-10-12, accepted in 2011-02-24,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundHuman bocavirus 2(HBoV2) and other human bocavirus species (HBoV, HBoV3, and HBoV4) have been discovered recently. But the precise phylogenetic relationships among these viruses are not clear yet.MethodsWe collected 632 diarrhea and 162 healthy children in Lanzhou, China. Using PCR, Human bocavirus (HBoV), HBoV2, HBoV3 and HBoV4 were screened. The partial genes of NS, NP1 and VP, and two nearly complete sequences of HBoV2 were obtained.ResultPhylogenetic analysis showed the different genes of HBoV2 strain were homogenous with different reference strains. HBoV3 may be a recombinant derived from HBoV and HBoV4. We also observed that the VP1 and VP2 region of HBoV3 is as similar to HBoV2 as to HBoV4.ConclusionsA single genetic lineage of HBoV2 is circulating in children with and without gastroenteritis in Lanzhou, China. Current evidence in this study was not enough to support recombination between HBoV2 strains, and HBoV3 may be a recombinant between HBoV and the common ancestor of HBoV2 and HBoV4.

【 授权许可】

Unknown   
© Cheng et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.