BDNF Val66Met polymorphism and protein levels in Amniotic Fluid

BMC Neuroscience

Research Article

Marco Andrea Riva¹  Francesca Calabrese¹  Daniela Bellotti²  Eleonora Marchina²  Sergio Barlati²  Stefania Moraschi³  Annamaria Cattaneo³  Elena Milanesi⁴  Massimo Gennarelli⁴  Luisella Bocchio-Chiavetto⁵  Roberta Zanardini⁵ 

[1] Center of Neuropharmacology, Department of Pharmacological Sciences and Center of Excellence on Neurodegenerative Diseases, University of Milan, Italy;Division of Biology and Genetics, Department of Biomedical Sciences and Biotechnology, University of Brescia, Italy;Genetics Unit, IRCCS San Giovanni di Dio, Fatebenefratelli, Brescia, Italy;Genetics Unit, IRCCS San Giovanni di Dio, Fatebenefratelli, Brescia, Italy;Division of Biology and Genetics, Department of Biomedical Sciences and Biotechnology, University of Brescia, Italy;Neuropsychopharmacology Unit, IRCCS San Giovanni di Dio, Fatebenefratelli, Brescia, Italy;

关键词: Amniotic Fluid;    BDNF Level;    Early Life Stress;    Central Nervous System Development;    Val66Met Polymorphism;   

DOI  :  10.1186/1471-2202-11-16

 received in 2009-06-12, accepted in 2010-02-08,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundBrain-Derived Neurotrophic Factor (BDNF) is a neurotrophin which plays survival- and growth-promoting activity in neuronal cells and it is involved in cellular plasticity mechanisms as it controls activity dependent synaptic transmission. A functional polymorphism (Val66Met) in the pro-region of BDNF, which affects the intracellular trafficking of proBDNF has been associated with memory and cognitive deficits as well as to an increased susceptibility for several psychiatric disorders especially those with a neurodevelopmental origin. To date, no study has evaluated the influence of the Val66Met polymorphism on BDNF levels in a peripheral system that may reflect fetal neurodevelopment. Therefore we investigated in amniotic fluids (AF) obtained from 139 healthy women during 15-17 week of pregnancy, BDNF protein levels in correlation with the Val66Met polymorphism.ResultsInterestingly we found a significant BDNF protein levels reduction in 55 Met carriers (Val/Met and Met/Met) (p = 0.002) as compared to 84 non carriers (Val/Val), and no effect of fetus gender, maternal age or gestation week on BDNF levels has been observed.ConclusionThese results, although explorative, indicate that during fetal life the Val66Met genotype might influences BDNF protein levels in AF supporting the involvement of this polymorphism in behavioral and functional brain individual differences in the adulthood.

【 授权许可】

Unknown   
© Cattaneo et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.