Induction of ROS generation and NF-κB activation in MARC-145 cells by a novel porcine reproductive and respiratory syndrome virus in Southwest of China isolate

BMC Veterinary Research

Research Article

Qian Liu¹  Yongke Sun¹  Zhiyong Shao¹  Yating Zang¹  Ling Chen¹  Hong Gao¹  Hui Huang¹  Yulin Yan²  Aiguo Xin³ 

[1] Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan, People’s Republic of China;Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan, People’s Republic of China;State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, People’s Republic of China;Yunnan Animal Science and Veterinary Institute, Kunming, Yunnan, People’s Republic of China;

关键词: PRRSV;    ROS;    NF-κB;    MARC-145 cells;   

DOI  :  10.1186/s12917-015-0480-z

 received in 2015-02-05, accepted in 2015-07-13,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundPorcine reproductive and respiratory syndrome virus (PRRSV) is the cause of an economically important swine disease that has devastated the swine industry since the late 1980s. The aim of the present study was to investigate the interaction between reactive oxygen species (ROS) and NF-κB by PRRSV infection.ResultsWe isolated the local strain of PRRSV from southwest China, designated YN-2011, then sequenced and analyzed the genome. YN-2011 was then used to evaluate the interaction of ROS and NF-κB. In PRRSV infected MARC-145 cells, there was a time-dependent increase in ROS and Maleic Dialdehyde (MDA). Accordingly, NF-κB activation was also increased as PRRSV infection progressed. Degradation of IκB mRNA was detected late in PRRSV infection, and overexpression of the dominant negative form of IκBα significantly suppressed NF-κB induced by PRRSV.ConclusionsThe results indicate that the generation of ROS is involved in PRRSV replication and this progression is associated with the alteration in NF-κB activity induced by ROS. These results should extend our better understanding the interaction between PRRSV and host MARC-145 cells.

【 授权许可】

CC BY   
© Yan et al. 2016