Specific antibody responses against membrane proteins of erythrocytes infected by Plasmodium falciparum of individuals briefly exposed to malaria

Malaria Journal

Research

Maya Belghazi¹  Matthieu Pophillat²  Patrick Fourquet²  Didier Lefranc³  Claude Villard⁴  Lionel Almeras⁵  Stéphanie Bourdon⁵  Thierry Fusai⁵  Albin Fontaine⁵  Claude Durand⁵  Christophe Rogier⁵ 

[1] Centre d'Analyse Protéomique de Marseille (CAPM), Institut Jean Roche, Faculté de médecine Nord, Marseille, France;Centre d'Immunologie de Marseille Luminy (CIML), Institut National de la Santé et de la Recherche Médicale, Centre National de la Recherche Scientifique, Université de la Méditerranée, Parc Scientifique de Luminy, Case 906, 13288, Marseille Cedex 09, Marseille, France;Laboratoire d'immunologie EA2686, Faculté de Médecine, 1, Place de Verdun, 59045, Lille Cedex, France;Plateforme protéomique MapTimone, INSERM UMR 911 CRO2, Faculté de Pharmacie/Université de la Méditerranée, 27 Bd Jean Moulin, 13385, Marseille Cedex 05, France;Unité de recherche en biologie et épidémiologie parasitaires (URBEP) - UMR6236 - IFR48, Antenne Marseille de l'Institut de Recherche Biomédicale des Armées (IRBA), BP 60109, 13 262, Marseille Cedex 07, France;

关键词: Malaria;    Malaria Transmission;    Severe Malaria;    Blood Stage;    Parasite Blood Stage;   

DOI  :  10.1186/1475-2875-9-276

 received in 2010-04-22, accepted in 2010-10-11,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundPlasmodium falciparum infections could lead to severe malaria, principally in non-immune individuals as children and travellers from countries exempted of malaria. Severe malaria is often associated with the sequestration of P. falciparum-infected erythrocytes in deep micro-vascular beds via interactions between host endothelial receptors and parasite ligands expressed on the surface of the infected erythrocyte. Although, serological responses from individuals living in endemic areas against proteins expressed at surface of the infected erythrocyte have been largely studied, seldom data are available about the specific targets of antibody response from travellers.MethodsIn order to characterize antigens recognized by traveller sera, a comparison of IgG immune response against membrane protein extracts from uninfected and P. falciparum-infected red blood cells (iRBC), using immunoblots, was performed between non exposed individuals (n = 31) and briefly exposed individuals (BEI) (n = 38) to malaria transmission.ResultsImmune profile analysis indicated that eight protein bands from iRBC were significantly detected more frequently in the BEI group. Some of these antigenic proteins were identified by an original immuno-proteomic approach.ConclusionCollectively, these data may be useful to characterize the singular serological immune response against a primary malaria infection in individuals briefly exposed to transmission.

【 授权许可】

Unknown   
© Fontaine et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.