Differential gene expression of human chondrocytes cultured under short-term altered gravity conditions during parabolic flight maneuvers

Cell Communication and Signaling

Research

Markus Wehland¹  Stefan Riwaldt¹  Jessica Pietsch¹  Elisabeth Warnke¹  Manfred Infanger¹  Ganna Aleshcheva¹  Ruth Hemmersbach²  Thomas Juhl Corydon³  Daniela Grimm³  Xiao Ma³  Timo Frett⁴  Markus Braun⁵  Katrin Saar⁶  Norbert Hübner⁶  Herbert Schulz⁷ 

[1] Clinic for Plastic, Aesthetic and Hand Surgery, Otto-von-Guericke University, 39120, Magdeburg, Germany;DLR German Aerospace Center, Biomedical Research, Gravitational Biology, 51147, Köln, Germany;Department of Biomedicine, Aarhus University, Wilhelm Meyers Allé 4, DK-8000, Aarhus C, Denmark;German Aerospace Center (DLR), Institute of Aerospace Medicine, Biomedical Research, 51147, Köln, Germany;Institute for Molecular Physiology and Biotechnology of Plants (IMBIO), University of Bonn, Gravitational Biology Group, 53115, Bonn, Germany;Max-Delbrück-Center for Molecular Medicine, 13092, Berlin, Germany;Max-Delbrück-Center for Molecular Medicine, 13092, Berlin, Germany;University of Cologne, Cologne Center for Genomics (CCG), 50931, Cologne, Germany;

关键词: Chondrocytes;    Gene expression;    Microgravity;    Hypergravity;    Vibration;    Cytokines;   

DOI  :  10.1186/s12964-015-0095-9

 received in 2014-11-13, accepted in 2015-03-02,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundChondrocytes are the main cellular component of articular cartilage. In healthy tissue, they are embedded in a strong but elastic extracelluar matrix providing resistance against mechanical forces and friction for the joints. Osteoarthritic cartilage, however, disrupted by heavy strain, has only very limited potential to heal. One future possibility to replace damaged cartilage might be the scaffold-free growth of chondrocytes in microgravity to form 3D aggregates.ResultsTo prepare for this, we have conducted experiments during the 20th DLR parabolic flight campaign, where we fixed the cells after the first (1P) and the 31st parabola (31P). Furthermore, we subjected chondrocytes to isolated vibration and hypergravity conditions. Microarray and quantitative real time PCR analyses revealed that hypergravity regulated genes connected to cartilage integrity (BMP4, MMP3, MMP10, EDN1, WNT5A, BIRC3). Vibration was clearly detrimental to cartilage (upregulated inflammatory IL6 and IL8, downregulated growth factors EGF, VEGF, FGF17). The viability of the cells was not affected by the parabolic flight, but showed a significantly increased expression of anti-apoptotic genes after 31 parabolas. The IL-6 release of chondrocytes cultured under conditions of vibration was not changed, but hypergravity (1.8 g) induced a clear elevation of IL-6 protein in the supernatant compared with corresponding control samples.ConclusionTaken together, this study provided new insights into the growth behavior of chondrocytes under short-term microgravity.

【 授权许可】

Unknown   
© Wehland et al.; licensee BioMed Central. 2015. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.