Three-dimensional in vitro follicle growth: overview of culture models, biomaterials, design parameters and future directions

Reproductive Biology and Endocrinology

Review

James Goldfarb¹  Nina Desai¹  Anastasia Alex¹  Faten AbdelHafez¹  Tommaso Falcone¹  Anthony Calabro²  Aaron Fleischman² 

[1] Cleveland Clinic Fertility Center, Department of OB/GYN and Women's Health Institute, Cleveland Clinic Foundation, Cleveland, Ohio, USA;Department of Biomedical Engineering, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, Ohio, USA;

关键词: Granulosa Cell;    Calcium Alginate;    Primordial Follicle;    Secondary Follicle;    Follicle Growth;   

DOI  :  10.1186/1477-7827-8-119

 received in 2010-07-19, accepted in 2010-10-14,  发布年份 2010

来源: Springer

【 摘 要 】

In vitro ovarian follicle culture is a new frontier in assisted reproductive technology with tremendous potential, especially for fertility preservation. Folliculogenesis within the ovary is a complex process requiring interaction between somatic cell components and the oocyte. Conventional two-dimensional culture on tissue culture substrata impedes spherical growth and preservation of the spatial arrangements between oocyte and surrounding granulosa cells. Granulosa cell attachment and migration can leave the oocyte naked and unable to complete the maturation process. Recognition of the importance of spatial arrangements between cells has spurred research in to three-dimensional culture system. Such systems may be vital when dealing with human primordial follicles that may require as long as three months in culture. In the present work we review pertinent aspects of in vitro follicle maturation, with an emphasis on tissue-engineering solutions for maintaining the follicular unit during the culture interval. We focus primarily on presenting the various 3-dimensional culture systems that have been applied for in vitro maturation of follicle:oocyte complexes. We also try to present an overview of outcomes with various biomaterials and animal models and also the limitations of the existing systems.

【 授权许可】

CC BY   
© Desai et al; licensee BioMed Central Ltd. 2010