The complete genome sequence of Corynebacterium pseudotuberculosis FRC41 isolated from a 12-year-old girl with necrotizing lymphadenitis reveals insights into gene-regulatory networks contributing to virulence

BMC Genomics

Research Article

Peter Husemann¹  Jens Stoye¹  Alexander Goesmann²  Sebastian Jaenicke²  Jeronimo Ruiz³  Olivier F Join-Lambert⁴  Samer Kayal⁵  Nicole Guiso⁶  Andreas Tauch⁷  Jasmin Schröder⁷  Eva Trost⁸  Jessica Schneider⁹  Artur Silva^1,10  Vívian D'Afonseca^1,11  Anderson Miyoshi^1,11  Vasco Azevedo^1,11  Siomar de Castro Soares^1,11  Flavia Souza Rocha^1,11  Fernanda Alves Dorella^1,11  Lisa Ott^1,12  Andreas Burkovski^1,12 

[1] AG Genominformatik, Technische Fakultät, Universität Bielefeld, D-33615, Bielefeld, Universitätsstraße 25, Germany;Bioinformatics Resource Facility, Centrum für Biotechnologie, Universität Bielefeld, Universitätsstraße 27, D-33615, Bielefeld, Germany;Cellular and Molecular Parasitology Laboratory, Rene Rachou Research Center, Oswaldo Cruz Foundation (FIOCRUZ), Belo Horizonte, MG, Brazil;Department of Microbiology, Medical Faculty, University Paris Descartes, Hospital Necker Enfants Malades, 147 rue de Sèvres, 75015, Paris, France;Department of Microbiology, Medical Faculty, University Rennes 1, Hospital Pontchaillou, 2 rue Henri le Guilloux, 35000, Rennes, France;Institut Pasteur, Unité de Prévention et Thérapies Moléculaires des Maladies Humaines, National Centre of Reference of Toxigenic Corynebacteria, 25 rue du Dr. Roux, 75724, Paris Cedex 15, France;Institut für Genomforschung und Systembiologie, Centrum für Biotechnologie, Universität Bielefeld, Universitätsstraße 27, D-33615, Bielefeld, Germany;Institut für Genomforschung und Systembiologie, Centrum für Biotechnologie, Universität Bielefeld, Universitätsstraße 27, D-33615, Bielefeld, Germany;CLIB Graduate Cluster Industrial Biotechnology, Centrum für Biotechnologie, Universität Bielefeld, Universitätsstraße 27, D-33615, Bielefeld, Germany;Institut für Genomforschung und Systembiologie, Centrum für Biotechnologie, Universität Bielefeld, Universitätsstraße 27, D-33615, Bielefeld, Germany;CLIB Graduate Cluster Industrial Biotechnology, Centrum für Biotechnologie, Universität Bielefeld, Universitätsstraße 27, D-33615, Bielefeld, Germany;Bioinformatics Resource Facility, Centrum für Biotechnologie, Universität Bielefeld, Universitätsstraße 27, D-33615, Bielefeld, Germany;Instituto de Ciências Biológicas, Universidade Federal do Pará, Rua Augusto Corrêa, 01 - Guamá, Belém, PA, Brazil;Laboratório de Genética Celular e Molecular, Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Pampulha, Belo Horizonte, MG, Av. Antonio Carlos 6627, Brazil;Lehrstuhl für Mikrobiologie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Staudtstraße 5, D-91058, Erlangen, Germany;

关键词: Nonribosomal Peptide Synthetase;    Potential Virulence Factor;    Siderophore Biosynthesis;    Caseous Lymphadenitis;    Pilin Subunit;   

DOI  :  10.1186/1471-2164-11-728

 received in 2010-09-08, accepted in 2010-12-30,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundCorynebacterium pseudotuberculosis is generally regarded as an important animal pathogen that rarely infects humans. Clinical strains are occasionally recovered from human cases of lymphadenitis, such as C. pseudotuberculosis FRC41 that was isolated from the inguinal lymph node of a 12-year-old girl with necrotizing lymphadenitis. To detect potential virulence factors and corresponding gene-regulatory networks in this human isolate, the genome sequence of C. pseudotuberculosis FCR41 was determined by pyrosequencing and functionally annotated.ResultsSequencing and assembly of the C. pseudotuberculosis FRC41 genome yielded a circular chromosome with a size of 2,337,913 bp and a mean G+C content of 52.2%. Specific gene sets associated with iron and zinc homeostasis were detected among the 2,110 predicted protein-coding regions and integrated into a gene-regulatory network that is linked with both the central metabolism and the oxidative stress response of FRC41. Two gene clusters encode proteins involved in the sortase-mediated polymerization of adhesive pili that can probably mediate the adherence to host tissue to facilitate additional ligand-receptor interactions and the delivery of virulence factors. The prominent virulence factors phospholipase D (Pld) and corynebacterial protease CP40 are encoded in the genome of this human isolate. The genome annotation revealed additional serine proteases, neuraminidase H, nitric oxide reductase, an invasion-associated protein, and acyl-CoA carboxylase subunits involved in mycolic acid biosynthesis as potential virulence factors. The cAMP-sensing transcription regulator GlxR plays a key role in controlling the expression of several genes contributing to virulence.ConclusionThe functional data deduced from the genome sequencing and the extended knowledge of virulence factors indicate that the human isolate C. pseudotuberculosis FRC41 is equipped with a distinct gene set promoting its survival under unfavorable environmental conditions encountered in the mammalian host.

【 授权许可】

Unknown   
© Trost et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.