Deep sequencing-based transcriptome analysis of Plutella xylostella larvae parasitized by Diadegma semiclausum

BMC Genomics

Research Article

David Schlipalius¹  Robin W Palfreyman²  Lars K Nielsen²  Kayvan Etebari³  Sassan Asgari³  Richard V Glatz⁴ 

[1] AgriScience Queensland, Department of Employment Economic Development and Innovation, Ecosciences Precinct, GPO Box 46, 4001, Brisbane, Australia;Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, 4072, St Lucia, QLD, Australia;School of Biological Sciences, The University of Queensland, 4072, St Lucia, QLD, Australia;South Australian Research and Development Institute (SARDI), Entomology, Waite Road, 5064, Urrbrae, South Australia, Australia;

关键词: Deep Sequencing;    Juvenile Hormone;    Hemocyte;    Plutella Xylostella;    Parasitoid Attack;   

DOI  :  10.1186/1471-2164-12-446

 received in 2011-03-09, accepted in 2011-09-09,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundParasitoid insects manipulate their hosts' physiology by injecting various factors into their host upon parasitization. Transcriptomic approaches provide a powerful approach to study insect host-parasitoid interactions at the molecular level. In order to investigate the effects of parasitization by an ichneumonid wasp (Diadegma semiclausum) on the host (Plutella xylostella), the larval transcriptome profile was analyzed using a short-read deep sequencing method (Illumina). Symbiotic polydnaviruses (PDVs) associated with ichneumonid parasitoids, known as ichnoviruses, play significant roles in host immune suppression and developmental regulation. In the current study, D. semiclausum ichnovirus (Ds IV) genes expressed in P. xylostella were identified and their sequences compared with other reported PDVs. Five of these genes encode proteins of unknown identity, that have not previously been reported.ResultsDe novo assembly of cDNA sequence data generated 172,660 contigs between 100 and 10000 bp in length; with 35% of > 200 bp in length. Parasitization had significant impacts on expression levels of 928 identified insect host transcripts. Gene ontology data illustrated that the majority of the differentially expressed genes are involved in binding, catalytic activity, and metabolic and cellular processes. In addition, the results show that transcription levels of antimicrobial peptides, such as gloverin, cecropin E and lysozyme, were up-regulated after parasitism. Expression of ichnovirus genes were detected in parasitized larvae with 19 unique sequences identified from five PDV gene families including vankyrin, viral innexin, repeat elements, a cysteine-rich motif, and polar residue rich protein. Vankyrin 1 and repeat element 1 genes showed the highest transcription levels among the Ds IV genes.ConclusionThis study provides detailed information on differential expression of P. xylostella larval genes following parasitization, Ds IV genes expressed in the host and also improves our current understanding of this host-parasitoid interaction.

【 授权许可】

Unknown   
© Etebari et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.