Differential expression of NBS-LRR-encoding genes in the root transcriptomes of two Solanum phureja genotypes with contrasting resistance to Globodera rostochiensis

BMC Plant Biology

Research

Olga S. Afanasenko¹  Alexander Khiutti¹  Nikolay A. Shmakov²  Dmitry A. Afonnikov²  Natalja V. Shatskaya²  Gennady V. Vasilyev²  Kseniya V. Strygina²  Salmaz M. Ibragimova²  Sophia V. Gerasimova²  Anastasiya Y. Glagoleva³  Elena K. Khlestkina³  Alex V. Kochetov⁴  Olga Y. Antonova⁵  Natalia V. Alpatyeva⁵  Tatyana A. Gavrilenko⁶ 

[1] All Russian Research Institute for Plant Protection, 196608, Saint Petersburg, Russia;Institute of Cytology and Genetics, SB RAS, 630090, Novosibirsk, Russia;Institute of Cytology and Genetics, SB RAS, 630090, Novosibirsk, Russia;Novosibirsk State University, 630090, Novosibirsk, Russia;Institute of Cytology and Genetics, SB RAS, 630090, Novosibirsk, Russia;Novosibirsk State University, 630090, Novosibirsk, Russia;Novosibirsk State Agrarian University, 630039, Novosibirsk, Russia;Vavilov Institute of Plant Genetic Resources (VIR), 190000, Saint Petersburg, Russia;Vavilov Institute of Plant Genetic Resources (VIR), 190000, Saint Petersburg, Russia;St. Petersburg State University, 199034, St. Petersburg, Russia;

关键词: NBS-LRR genes;    Solanum phureja;    Resistance;    Globodera rostochiensis;   

DOI  :  10.1186/s12870-017-1193-1

来源: Springer

【 摘 要 】

BackgroundThe characterization of major resistance genes (R genes) in the potato remains an important task for molecular breeding. However, R genes are rapidly evolving and frequently occur in genomes as clusters with complex structures, and their precise mapping and identification are complicated and time consuming.ResultsComparative analysis of root transcriptomes of Solanum phureja genotypes with contrasting resistance to Globodera rostochiensis revealed a number of differentially expressed genes. However, compiling a list of candidate R genes for further segregation analysis was hampered by their scarce annotation. Nevertheless, combination of transcriptomic analysis with data on predicted potato NBS-LRR-encoding genes considerably improved the quality of the results and provided a reasonable number of candidate genes that provide S. phureja with strong resistance to the potato golden cyst nematode.ConclusionCombination of comparative analyses of tissue-specific transcriptomes in resistant and susceptible genotypes may be used as an approach for the rapid identification of candidate potato R genes for co-segregation analysis and may be used in parallel with more sophisticated studies based on genome resequencing.

【 授权许可】

CC BY   
© The Author(s). 2017