Development of a genetic tool for product regulation in the diverse British pig breed market

BMC Genomics

Research Article

Martien AM Groenen¹  Richard PMA Crooijmans¹  Hendrik-Jan Megens¹  Pamela Wiener²  Alan L Archibald²  Chris S Haley³  Samantha Wilkinson⁴  Rob Ogden⁵ 

[1] Animal Breeding and Genomics Centre, Wageningen University, Wageningen, The Netherlands;The Roslin Institute and (Royal) Dick School of Veterinary Studies, University of Edinburgh, EH25 9RG, Easter Bush, Midlothian, UK;The Roslin Institute and (Royal) Dick School of Veterinary Studies, University of Edinburgh, EH25 9RG, Easter Bush, Midlothian, UK;MRC Human Genetics Unit, Western General Hospital, Crewe Road, EH4 2XU, Edinburgh, UK;The Roslin Institute and (Royal) Dick School of Veterinary Studies, University of Edinburgh, EH25 9RG, Easter Bush, Midlothian, UK;Scotland's Rural College, The Roslin Institute Building, EH25 9RG, Easter Bush, Midlothian, UK;Wildgenes Laboratory, Royal Zoological Society of Scotland, EH12 6TS, EdinburghScotland, UK;

关键词: Single Nucleotide Polymorphism Marker;    Correct Assignment;    Traditional Breed;    Pork Product;    Breed Origin;   

DOI  :  10.1186/1471-2164-13-580

 received in 2011-10-13, accepted in 2012-03-21,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundThe application of DNA markers for the identification of biological samples from both human and non-human species is widespread and includes use in food authentication. In the food industry the financial incentive to substituting the true name of a food product with a higher value alternative is driving food fraud. This applies to British pork products where products derived from traditional pig breeds are of premium value. The objective of this study was to develop a genetic assay for regulatory authentication of traditional pig breed-labelled products in the porcine food industry in the United Kingdom.ResultsThe dataset comprised of a comprehensive coverage of breed types present in Britain: 460 individuals from 7 traditional breeds, 5 commercial purebreds, 1 imported European breed and 1 imported Asian breed were genotyped using the PorcineSNP60 beadchip. Following breed-informative SNP selection, assignment power was calculated for increasing SNP panel size. A 96-plex assay created using the most informative SNPs revealed remarkably high genetic differentiation between the British pig breeds, with an average FST of 0.54 and Bayesian clustering analysis also indicated that they were distinct homogenous populations. The posterior probability of assignment of any individual of a presumed origin actually originating from that breed given an alternative breed origin was > 99.5% in 174 out of 182 contrasts, at a test value of log(LR) > 0. Validation of the 96-plex assay using independent test samples of known origin was successful; a subsequent survey of market samples revealed a high level of breed label conformity.ConclusionThe newly created 96-plex assay using selected markers from the PorcineSNP60 beadchip enables powerful assignment of samples to traditional breed origin and can effectively identify mislabelling, providing a highly effective tool for DNA analysis in food forensics.

【 授权许可】

Unknown   
© 2012 Wilkinson et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.