Diphenyl diselenide derivatives inhibit microbial biofilm formation involved in wound infection

BMC Microbiology

Research Article

Rita Pagiotti¹  Luca Sancineto¹  Claudio Santi¹  Miranda Piccioni¹  Stefania De Marco¹  Donatella Pietrella¹  Vanessa Nascimento²  Antonio Luiz Braga² 

[1] Department of Pharmaceutical Sciences, University of Perugia, Via del Giochetto, 06122, Perugia, Italy;Laboratorio de Sıntese de Substancias de Selenio Bioativas, Centro de Ciencias Fısicas e Matematicas, Departamento de Quımica, Universidade Federal de Santa Catarina, 88040-900, Florianopolis, SC, Brazil;

关键词: Minimum Inhibitory Concentration;    DPPH;    Diselenides;    Antibiofilm Activity;    Organoselenium Compound;   

DOI  :  10.1186/s12866-016-0837-x

 received in 2016-05-24, accepted in 2016-09-13,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundOrganoselenium compounds have antimicrobial activity against some bacteria and fungi; furthermore, the antioxidant activity of diselenides has been demonstrated. The aim of the present work was to examine the in vitro minimal inhibitory concentration of a panel of differently substituted diselenides and their effectiveness in inhibiting biofilm formation and dispersing preformed microbial biofilm of Staphylococcus epidermidis, Staphylococcus aureus, Streptococcus pyogenes and Pseudomonas aeruginosa and the yeast Candida albicans, all involved in wound infections. Moreover, the cytotoxicity of the compounds was determined in human dermal fibroblast and keratinocytes. In closing, we tested their direct antioxidant activity.ResultsDiselenides showed different antimicrobial activity, depending on the microorganism. All diselenides demonstrated a good antibiofilm activity against S. aureus and S. epidermidis, the compounds camphor diselenide, bis[ethyl-N-(2’-selenobenzoyl) glycinate] and bis[2’-seleno-N-(1-methyl-2-phenylethyl) benzamide] were active against S. pyogenes and C. albicans biofilm while only diselenides 2,2’-diselenidyldibenzoic acid and bis[ethyl-N-(2’-selenobenzoyl) glycinate] were effective against P. aeruginosa. Moreover, the compounds bis[ethyl-N-(2’-selenobenzoyl) glycinate] and bis[2’-seleno-N-(1-methyl-2-phenylethyl) benzamide] showed an antioxidant activity at concentrations lower than the 50 % of cytotoxic concentration.ConclusionsBecause microbial biofilms are implicated in chronic infection of wounds and treatment failure, the combination of antimicrobial activity and potential radical scavenging effects may contribute to the improvement of wound healing. Therefore, this study suggests that bis[ethylN-(2’-selenobenzoyl) glycinate] and bis[2’-seleno-N-(1-methyl-2-phenylethyl) benzamide] are promising compounds to be used in preventing and treating microbial wound infections.

【 授权许可】

CC BY   
© The Author(s). 2016