The emergence of a novel sequence type of MDR Acinetobacter baumannii from the intensive care unit of an Egyptian tertiary care hospital

Annals of Clinical Microbiology and Antimicrobials

Research

Doaa Mohammad Ghaith¹  Mai Mahmoud Zafer²  Omar Almoazzamy³  Mohamed Hamed Al-Agamy⁴  Rayan Y. Booq⁵  Essam J. Alyamani⁵ 

[1] Department of Clinical and Chemical Pathology, Faculty of Medicine, Cairo University, Cairo, Egypt;Department of Microbiology and Immunology, Faculty of Pharmacy, Ahram Canadian University, Giza, Egypt;Department of Microbiology, Faculty of Science, Zagazig University, Zagazig, Egypt;Department of Pharmaceutics, College of Pharmacy, King Saud University, PO box 2457, 11451, Riyadh, Saudi Arabia;Department of Microbiology and Immunology, Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt;National Center for Biotechnology, King Abdulaziz City for Science and Technology, Riyadh, Saudi Arabia;

关键词: A. baumannii;    bla;    MLST;   

DOI  :  10.1186/s12941-017-0208-y

 received in 2016-12-14, accepted in 2017-04-12,  发布年份 2017

来源: Springer

【 摘 要 】

Background and aim of workAcinetobacter baumannii is known for nosocomial outbreaks worldwide. In this study, we aimed to investigate the antibiotic susceptibility patterns and the clonal relationship of A. baumannii isolates from the intensive care unit (ICU) of an Egyptian hospital.MethodsIn the present study, 50 clinical isolates of multidrug resistant (MDR)-A. baumannii were obtained from patients admitted into the ICU from June to December 2015. All isolates were analyzed for antimicrobial susceptibilities. Multiplex PCR was performed to detect genes encoding oxacillinase genes (blaOXA-51-like, blaOXA-23-like, blaOXA-24-like, and blaOXA-58-like). Multilocus sequence typing (MLST) based on the seven-gene scheme (gltA, gyrB, gdhB, recA, cpn60, gpi, rpoD) was used to examine these isolates.ResultsAll A. baumannii clinical isolates showed the same resistance pattern, characterized by resistance to most common antibiotics including imipenem (MIC ≥ 8μ/mL), with the only exception being colistin. Most isolates were positive for blaOXA-51-like and blaOXA-23-like (100 and 96%, respectively); however, blaOXA-24-like and blaOXA-58-like were not detected. MLST analysis identified different sequence types (ST195, ST208, ST231, ST441, ST499, and ST723) and a new sequence type (ST13929) with other sporadic strains.ConclusionsMDR A. baumannii strains harboring blaOXA-23-like genes were widely circulating in this ICU. MLST was a powerful tool for identifying and epidemiologically typing our strains. Strict infection control measures must be implemented to contain the worldwide spread of MDR A. baumannii in ICUs.

【 授权许可】

CC BY   
© The Author(s) 2017