Vitamin C further improves the protective effect of GLP-1 on the ischemia-reperfusion-like effect induced by hyperglycemia post-hypoglycemia in type 1 diabetes

Cardiovascular Diabetology

Original Investigation

Loredana Bucciarelli¹  Maurizio Rondinelli¹  Stefano Genovese¹  Antonio Ceriello²  Silvia Canivell²  Emilio Ortega²  Gemma Pujadas²  Lucia La Sala²  Anna Novials² 

[1] Department of Cardiovascular and Metabolic Diseases, IRCCS Gruppo Multimedica, Sesto San Giovanni, MI, Italy;Diabetes and Endocrinology, Insititut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), Hospital Clínic Barcelona, C/Rosselló, 149-153, 08036, Barcelona, Spainn;

关键词: Diabetes Mellitus;    Hypoglycemia;    Hyperglycemia;    Endothelial Dysfunction;    Oxidative Stress;    GLP-1;   

DOI  :  10.1186/1475-2840-12-97

 received in 2013-05-20, accepted in 2013-06-23,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundIt has been reported that hyperglycemia following hypoglycemia produces an ischemia-reperfusion-like effect in type 1 diabetes. In this study the possibility that GLP-1 has a protective effect on this phenomenon has been tested.Methods15 type 1 diabetic patients underwent to five experiments: a period of two hours of hypoglycemia followed by two hours of normo-glycemia or hyperglycemia with the concomitant infusion of GLP-1 or vitamin C or both. At baseline, after 2 and 4 hours, glycemia, plasma nitrotyrosine, plasma 8-iso prostaglandin F2alpha, sCAM-1a, IL-6 and flow mediated vasodilation were measured.ResultsAfter 2 h of hypoglycemia, flow mediated vasodilation significantly decreased, while sICAM-1, 8-iso-PGF2a, nitrotyrosine and IL-6 significantly increased. While recovering with normoglycemia was accompanied by a significant improvement of endothelial dysfunction, oxidative stress and inflammation, a period of hyperglycemia after hypoglycemia worsens all these parameters. These effects were counterbalanced by GLP-1 and better by vitamin C, while the simultaneous infusion of both almost completely abolished the effect of hyperglycemia post hypoglycemia.ConclusionsThis study shows that GLP-1 infusion, during induced hyperglycemia post hypoglycemia, reduces the generation of oxidative stress and inflammation, improving the endothelial dysfunction, in type 1 diabetes. Furthermore, the data support that vitamin C and GLP-1 may have an additive protective effect in such condition.

【 授权许可】

CC BY   
© Ceriello et al.; licensee BioMed Central Ltd. 2013