Assessment of Anopheles salivary antigens as individual exposure biomarkers to species-specific malaria vector bites

Malaria Journal

Research

Frederic Pagès¹  Franck Remoué²  Stephane Audebert³  Yvan Boublik⁴  Nawal Bakkali⁵  Zakia M I Ali⁵  Albin Fontaine⁵  Christophe Fraisier⁵  Lionel Almeras⁶  Vinh Vu Hai⁶  Mahfoud Bakli⁶  Christophe Rogier⁷ 

[1] Institut de Recherche Biomédicale des Armées (IRBA), antenne Marseille, Unité d’Entomologie Médicale, URMITE UMR 6236, GSBdD de Marseille Aubagne, BP 40026, 111 avenue de la corse, 13568, Marseille cedex 02, France;Cire Océan indien, 2bis avenue Georges Brassens, CS 60050-97408, Saint Denis Cedex 9, LA REUNION;Laboratoire Maladies Infectieuses et Vecteurs: Ecologie, UMR 224 CNRS/IRD/UM1, Génétique, Evolution et Contrôle, Montpellier, France;Marseille Proteomic (MaP), Centre de Recherche en Cancérologie de Marseille (CRCM), INSERM CNRS, Aix-Marseille Université, BP 30059, Institut Paoli-Calmette, 27, boulevard Leï Roure, 13273, Marseille cedex 09, France;UMR5237, CNRS/CRBM, F-34293, Montpellier, France;Unité de recherche en biologie et épidémiologie parasitaires (URBEP) – UMR63 – IFR48, Institut de Recherche Biomédicale des Armées (Armed forces biomedical research institute, IRBA), antenne Marseille, GSBdD de Marseille Aubagne, BP 40026, 111 avenue de la corse, 13568, Marseille cedex 02, France;Unité de recherche en biologie et épidémiologie parasitaires (URBEP) – UMR63 – IFR48, Institut de Recherche Biomédicale des Armées (Armed forces biomedical research institute, IRBA), antenne Marseille, GSBdD de Marseille Aubagne, BP 40026, 111 avenue de la corse, 13568, Marseille cedex 02, France;Aix Marseille Université, Unité de Recherche en Maladies Infectieuses et Tropicales Emergentes (URMITE), UM63, CNRS 7278, IRD 198, Inserm 1095, WHO collaborative center for rickettsioses and other arthropod borne bacterial diseases, Faculté de Médecine, 27 bd Jean Moulin, 13385, Marseille cedex 5, France;Unité de recherche en biologie et épidémiologie parasitaires (URBEP) – UMR63 – IFR48, Institut de Recherche Biomédicale des Armées (Armed forces biomedical research institute, IRBA), antenne Marseille, GSBdD de Marseille Aubagne, BP 40026, 111 avenue de la corse, 13568, Marseille cedex 02, France;Institut Pasteur de Madagascar, B.P. 1274, Ambohitrakely, 101, Antananarivo, Madagascar;

关键词: Anopheles gambiae;    Anopheles funestus;    Antigenic salivary proteins;    SG6;    5′nucleotidase;    Exposure biomarkers;    Epidemiology;   

DOI  :  10.1186/1475-2875-11-439

 received in 2012-10-22, accepted in 2012-12-19,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundMalaria transmission occurs during the blood feeding of infected anopheline mosquitoes concomitant with a saliva injection into the vertebrate host. In sub-Saharan Africa, most malaria transmission is due to Anopheles funestus s.s and to Anopheles gambiae s.l. (mainly Anopheles gambiae s.s. and Anopheles arabiensis). Several studies have demonstrated that the immune response against salivary antigens could be used to evaluate individual exposure to mosquito bites. The aim of this study was to assess the use of secreted salivary proteins as specific biomarkers of exposure to An. gambiae and/or An. funestus bites.MethodsFor this purpose, salivary gland proteins 6 (SG6) and 5′nucleotidases (5′nuc) from An. gambiae (gSG6 and g-5′nuc) and An. funestus (fSG6 and f-5′nuc) were selected and produced in recombinant form. The specificity of the IgG response against these salivary proteins was tested using an ELISA with sera from individuals living in three Senegalese villages (NDiop, n = 50; Dielmo, n = 38; and Diama, n = 46) that had been exposed to distinct densities and proportions of the Anopheles species. Individuals who had not been exposed to these tropical mosquitoes were used as controls (Marseille, n = 45).ResultsThe IgG responses against SG6 recombinant proteins from these two Anopheles species and against g-5′nucleotidase from An. gambiae, were significantly higher in Senegalese individuals compared with controls who were not exposed to specific Anopheles species. Conversely, an association was observed between the level of An. funestus exposure and the serological immune response levels against the f-5′nucleotidase protein.ConclusionThis study revealed an Anopheles salivary antigenic protein that could be considered to be a promising antigenic marker to distinguish malaria vector exposure at the species level. The epidemiological interest of such species-specific antigenic markers is discussed.

【 授权许可】

Unknown   
© Ali et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.