The ears of the African elephant: unexpected high seroprevalence of Plasmodium ovale and Plasmodium malariae in healthy populations in Western Africa

Malaria Journal

Research

André Bigot¹  Ludovic Anani¹  Denis Filisetti²  Julie Brunet²  Lydia Meckert²  Alexander W Pfaff²  Sylvie Perrotey²  Ermanno Candolfi²  Cécile Doderer-Lang²  Ahmed Aboubacar²  Elodie Haar²  Nicodème W Chabi³  Pascal S Atchade³  Casimir D Akpovi³  Ambaliou Sanni³ 

[1] Agence Nationale pour la Transfusion Sanguine (Ministère de la Santé), 01 B.P. 511, Cotonou, Bénin;Institut de Parasitologie et de Pathologie Tropicale Université de Strasbourg, 3, Rue Koeberlé, F67000, Strasbourg, France;Laboratoire de Biochimie et de Biologie Moléculaire, Université d’Abomey Calavi, 04 BP 0320, Cotonou, Bénin;

关键词: Plasmodium;    Seroprevalence;    Recombinant protein;    ELISA;    Africa;   

DOI  :  10.1186/1475-2875-13-240

 received in 2014-03-21, accepted in 2014-05-29,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundMalaria Is A Life-Threatening Pathology In Africa. Plasmodium Falciparum And Plasmodium Vivax Attract The Most Focus Because Of Their High Prevalence And Mortality. Knowledge About The Prevalence Of The Cryptic Pathogens Plasmodium Ovale And Plasmodium Malariae Is Limited. Thanks To Recombinant Tools, Their Seroprevalence Was Measured For The First Time, As Well As The Prevalence Of Mixed Infections In A Malaria-Asymptomatic Population In Benin, A Malaria-Endemic Country.MethodsA Panel Of 1,235 Blood Donations Collected Over Ten Months In Benin Was Used For Validation Of The Recombinant Tools. Recombinant P. Falciparum, P. Malariae, P. Ovale MSP1, And P. Falciparum AMA1 Were Engineered And Validated On A Biobank With Malaria-Infected Patients (N = 144) Using A Species-Speific ELISA Test (Recelisa). Results Were Compared To An ELISA Using A Native P. Falciparum Antigen (NatELISA).ResultsAmong Microscopically Negative African Blood Donors, 85% (1,050/1,235) Present Antibodies Directed To Native P. Falciparum, 94.4% (1,166/1,235) To rPf MSP1 And rPf AMA1, 56.8% (702/1,235) To rPo MSP1, 67.5% (834/1235) To rPm MSP1 And 45.3% Of The Malaria Seropositive Population Had Antibodies Recognizing The Three Species.ConclusionA High Rate Of Antibodies Against P. Ovale And P. Malariae Was Found In Asymptomatic Blood Donors. The Proportion Of Mixed Infections Involving Three Species Was Also Unexpected. These Data Suggest That Determining Seroprevalence For These Cryptic Species Is An Appropriate Tool To Estimate Their Incidence, At The Eve Of Upcoming Anti-P. Falciparum Vaccination Campaigns.

【 授权许可】

CC BY   
© Doderer-Lang et al.; licensee BioMed Central Ltd. 2014