Reduced graphene oxide induces transient blood–brain barrier opening: an in vivo study

Journal of Nanobiotechnology

Research

Edilene Siqueira Soares¹  Marcelo Bispo de Jesus¹  Rodrigo Ramos Catharino²  Mônica Siqueira Ferreira²  Monique Culturato Padilha Mendonça³  Maria Alice da Cruz-Höfling³  Helder José Ceragioli⁴ 

[1] Department of Biochemistry and Tissue Biology, Institute of Biology, State University of Campinas, Campinas, SP, Brazil;Department of Medicine and Experimental Surgery, Faculty of Medical Sciences, State University of Campinas, Campinas, SP, Brazil;Department of Pharmacology, Faculty of Medical Sciences, State University of Campinas, Campinas, SP, Brazil;Department of Biochemistry and Tissue Biology, Institute of Biology, State University of Campinas, Campinas, SP, Brazil;Department of Semiconductors, Instruments and Photonics, Faculty of Electrical and Computer Engineering, State University of Campinas, Campinas, SP, Brazil;

关键词: Blood–brain barrier;    Paracellular pathway;    Nanomaterials;    MALDI-MSI;   

DOI  :  10.1186/s12951-015-0143-z

 received in 2015-09-07, accepted in 2015-10-27,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundThe blood–brain barrier (BBB) is a complex physical and functional barrier protecting the central nervous system from physical and chemical insults. Nevertheless, it also constitutes a barrier against therapeutics for treating neurological disorders. In this context, nanomaterial-based therapy provides a potential alternative for overcoming this problem. Graphene family has attracted significant interest in nanomedicine because their unique physicochemical properties make them amenable to applications in drug/gene delivery and neural interface.ResultsIn this study, reduced graphene oxide (rGO) systemically-injected was found mainly located in the thalamus and hippocampus of rats. The entry of rGO involved a transitory decrease in the BBB paracellular tightness, as demonstrated at anatomical (Evans blue dye infusion), subcellular (transmission electron microscopy) and molecular (junctional protein expression) levels. Additionally, we examined the usefulness of matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) as a new imaging method for detecting the temporal distribution of nanomaterials throughout the brain.ConclusionsrGO was able to be detected and monitored in the brain over time provided by a novel application for MALDI-MSI and could be a useful tool for treating a variety of brain disorders that are normally unresponsive to conventional treatment because of BBB impermeability.

【 授权许可】

CC BY   
© Mendonça et al. 2015