Changes in concentrations of haemostatic and inflammatory biomarkers in synovial fluid after intra-articular injection of lipopolysaccharide in horses

BMC Veterinary Research

Research Article

Anne Mette Lindberg Vinther¹  Stine Mandrup Andreassen¹  Stine Jacobsen¹  Aziz Tnibar¹  Pia Haubro Andersen²  Annemarie T. Kristensen³  Søren Saxmose Nielsen⁴ 

[1] Department of Veterinary Clinical Sciences, Section of Large Animal Medicine and Surgery, University of Copenhagen, Højbakkegård Allé 5, DK-2630, Tåstrup, Denmark;Department of Veterinary Clinical Sciences, Section of Large Animal Medicine and Surgery, University of Copenhagen, Højbakkegård Allé 5, DK-2630, Tåstrup, Denmark;Department of Clinical Sciences, Swedish Agricultural University, 75007, Uppsala, Sweden;Department of Veterinary Clinical Sciences, University of Copenhagen, Dyrlægevej 16, DK-1870, Frederiksberg C, Denmark;Department of Veterinary and Animal Sciences, University of Copenhagen, Grønnegårdsvej 8, DK-1870, Frederiksberg C, Denmark;

关键词: Equine;    Acute arthritis;    Haemostasis;    Inflammation;    Thrombin-antithrombin;    d-dimer;    Fibrinogen;    Haptoglobin;    Iron;    Serum amyloid A;   

DOI  :  10.1186/s12917-017-1089-1

 received in 2016-02-19, accepted in 2017-06-01,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundSeptic arthritis is a common and potentially devastating disease characterized by severe intra-articular (IA) inflammation and fibrin deposition. Research into equine joint pathologies has focused on inflammation, but recent research in humans suggests that both haemostatic and inflammatory pathways are activated in the joint compartment in arthritic conditions. The aim of this study was to characterize the IA haemostatic and inflammatory responses in horses with experimental lipopolysaccharide (LPS)-induced joint inflammation. Inflammation was induced by IA injection of LPS into one antebrachiocarpal joint of six horses. Horses were evaluated clinically with subjective grading of lameness, and blood and synovial fluid (SF) samples were collected at post injection hours (PIH) -120, −96, −24, 0, 2, 4, 8, 16, 24, 36, 48, 72 and 144. Total protein (TP), white blood cell counts (WBC), serum amyloid A (SAA), haptoglobin, iron, fibrinogen, thrombin-antithrombin (TAT) and d-dimer concentrations were assessed in blood and SF.ResultsIntra-articular injection of LPS caused local and systemic signs of inflammation including increased rectal temperature, lameness and increased joint circumference and skin temperature. Most of the biomarkers (TP, WBC, haptoglobin, fibrinogen and TAT) measured in SF increased quickly after LPS injection (at PIH 2–4), whereas SAA and d-dimer levels increased more slowly (at PIH 16 and 144, respectively). SF iron concentrations did not change statistically significantly. Blood WBC, SAA, haptoglobin and fibrinogen increased and iron decreased significantly in response to the IA LPS injection, while TAT and d-dimer concentrations did not change. Repeated pre-injection arthrocenteses caused significant changes in SF concentrations of TP, WBC and haptoglobin.ConclusionSimilar to inflammatory joint disease in humans, joint inflammation in horses was accompanied by an IA haemostatic response with changes in fibrinogen, TAT and d-dimer concentrations. Inflammatory and haemostatic responses were induced simultaneously and may likely interact. Further studies of interactions between the two responses are needed for a better understanding of pathogenesis of joint disease in horses. Knowledge of effects of repeated arthrocenteses on levels of SF biomarkers may be of value when markers are used for diagnostic purposes.

【 授权许可】

CC BY   
© The Author(s). 2017