Sperm motility and morphology changes in rats exposed to cadmium and diazinon

Reproductive Biology and Endocrinology

Research

Peter Massanyi¹  Vladimira Krajcovicova²  Maria Adamkovicova²  Radoslav Omelka²  Robert Toman³  Monika Martiniakova⁴  Ramona Babosova⁴  Birgit Grosskopf⁵ 

[1] Department of Animal Physiology, Slovak University of Agriculture, 949 76, Nitra, Slovakia;Department of Botany and Genetics, Constantine the Philosopher University, 949 74, Nitra, Slovakia;Department of Veterinary Disciplines, Slovak University of Agriculture, 949 76, Nitra, Slovakia;Department of Zoology and Anthropology, Constantine the Philosopher University, 949 74, Nitra, Slovakia;Institute of Zoology and Anthropology, Georg-August University, 37 073, Göttingen, Germany;

关键词: Sperm;    Motility;    Morphology;    Rat;    Cadmium;    Diazinon;   

DOI  :  10.1186/s12958-016-0177-6

 received in 2016-06-17, accepted in 2016-07-28,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundHumans are ubiquitously exposed to multiple environmental contaminants. Consequences of combined action on the reproductive system remain unknown. This study aimed to assess single and joint effects of cadmium and diazinon exposure on sperm quality parameters.MethodsMale adult Wistar rats were randomized into 4 groups of ten animals each. Group A was used as a control, animals from group B were exposed to cadmium (30 mg/L), rats from group C were administered with diazinon (40 mg/L), and rats from group D were exposed simultaneously to cadmium (30 mg/L) and diazinon (40 mg/L) via drinking water for 90 days. Sperm morphology and motility were evaluated using a bright field microscope and a computer-assisted semen analysis.ResultsThe percentage of motile spermatozoa and morphologically normal sperm was markedly reduced in rats from the group B. Rats from the C group showed an increase in velocity parameters, amplitude of lateral head displacement, decrease in beat-cross frequency, and an increase in abnormal sperm morphology. Simultaneous coexposure to cadmium and diazinon increased distance and velocity parameters, and amplitude of lateral head displacement. Reductions were observed in straightness, linearity, wobble, and beat-cross frequency. The decreased normal sperm morphology rates were related to defects of the sperm tail.ConclusionsExposure to cadmium and diazinon at relatively low doses impairs sperm quality and can reduce male fertility. Cadmium and diazinon caused significant changes on sperm morphology with varying effects on motility patterns. These parameters were significantly higher in the group D as compared to the group C. The findings have important implications for reproductive risk assessment of combined exposures to multiple chemicals.

【 授权许可】

CC BY   
© The Author(s). 2016