Gene polymorphism and protein of human pro- and anti-inflammatory cytokines in Chinese healthy subjects and chronic periodontitis patients

Journal of Translational Medicine

Proceedings

Lan-jun Bai¹  Mary NB Cheung²  Yi-ding Dou²  Yuan Yue³  Min Wang³  Jin-le Li³  Hao Liang³  Ye Tian³  Chang-bin Fan⁴  Liu Qing⁵  Louis WC Chow⁵  Wings TY Loo⁶ 

[1] Department of Stomatology, Sichuan Academy of Medical Sciences & Sichuan Provincial People’s Hospital, No.32, Section 2,1st Ring Road (West), Chengdu, Sichuan, PRC;Jin Hua Dentistry, 610041, Chengdu, Sichuan, PRC;State Key Laboratory for Oral Diseases and Department of Prosthodontics, West China Hospital of Stomatology, Sichuan University, Sichuan, PRC;Stomatological Hospital of Guangzhou Medical College, Guangzhou, PRC;UNIMED Medical Institute, Hong Kong, SAR;UNIMED Medical Institute, Hong Kong, SAR;School of Chinese Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, SAR;

关键词: Periodontitis;    Detection Frequency;    Chronic Periodontitis;    Gingival Crevicular Fluid;    Gingival Recession;   

DOI  :  10.1186/1479-5876-10-S1-S8

来源: Springer

【 摘 要 】

BackgroundPeriodontal disease is thought to arise from the interaction of various factors, including the susceptibility of the host, the presence of pathogenic organisms, and the absence of beneficial species. The genetic factors may play a significant role in the risk of periodontal diseases. Cytokines initiate, mediate and control immune and inflammatory responses. The aim of this study is to compare genotypes and soluble protein of pro and anti-inflammatory cytokines (IL-1α, IL-1β, IL-6, IFN-γ, IL-10, TNF-α and IL-4) in subjects with or free of chronic periodontitis.MethodsA total of 1,290 Chinese subjects were recruited to this clinical trial: 850 periodontally healthy controls and 440 periodontal patients. All subjects were free of systemic diseases. Oral examinations were performed, and the following parameters were recorded for each subject: supragingival/subgingival calculus, gingival recession, bleeding on probing (BOP), probing depth (PD), clinical attachment loss (CAL), gingival recession and tooth mobility. The peripheral blood samples were collected for genetic and enzyme linked immunosorbent assay (ELISA) analysis. Restriction enzymes were used for digestion of amplified fragments of IL-1α, IL-1β, IL-6, IFN-γ, IL-10, TNF-α and IL-4.ResultsThe protein expressions of patient and control samples for IL-1α, IL-1β, IL-6, TNF-α, IFN-γ, IL-10, and IL-4 measured by ELISA confirmed a statistically significant difference (p < 0.001). The digestion of fragments of various genes showed that the pro-inflammatory cytokines IL-1α and TNF-α, and the anti-inflammatory cytokines IL-4 and IL-10 demonstrated a correlation with chronic inflammation in patients (X2: p < 0.001). The remaining genes investigated in patients and healthy subjects (IL-1β, IL-6, IFN-γ and IL-10) did not show any significant difference.ConclusionsThe cytokine gene polymorphisms may be used as a marker for periodontitis susceptibility, clinical behaviour and severity. This detection offers early diagnosis and induction of prophylaxis to other family members against disease progression.

【 授权许可】

Unknown   
© Loo et al; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.