Asymptomatic infections with highly polymorphic Chlamydia suis are ubiquitous in pigs

BMC Veterinary Research

Research Article

Yvonne Pannekoek¹  Martina Jelocnik²  Adam Polkinghorne²  Bernhard Kaltenboeck³  Nicole Borel⁴  Zhixin Feng⁵  Ping Jiang⁶  Jianseng Gong⁷  Xin Zhou⁸  Jilei Zhang⁸  Jiawei Wang⁸  Yaoyao Wang⁸  Feng Yang⁸  Min Li⁸  Jing Li⁸  Chengming Wang⁹  Chunlian Song^1,10 

[1] Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands;Centre for Animal Health Innovation, Faculty of Science, Health, Education & Engineering, University of the Sunshine Coast, QLD, Maroochydore, Australia;College of Veterinary Medicine, Auburn University, Auburn, Alabama, USA;Institute for Veterinary Pathology, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland;Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences; Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, National Center for Engineering Research of Veterinary Bio-Products, Nanjing, China;Key Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China;Poultry Institute, Chinese Academy of Agricultural Sciences, Yangzhou, Jiangsu, China;Yangzhou University College of Veterinary Medicine, Yangzhou, Jiangsu, People’s Republic of China;Yangzhou University College of Veterinary Medicine, Yangzhou, Jiangsu, People’s Republic of China;College of Veterinary Medicine, Auburn University, Auburn, Alabama, USA;Yunnan Agricultural University College of Animal Science & Technology, Kunming, Yunnan, China;

关键词: Chlamydia suis;    Pig;    FRET-PCR;    ompA;    MLST;    Tanglegram;   

DOI  :  10.1186/s12917-017-1295-x

 received in 2017-07-03, accepted in 2017-11-22,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundChlamydia suis is an important, globally distributed, highly prevalent and diverse obligate intracellular pathogen infecting pigs. To investigate the prevalence and genetic diversity of C. suis in China, 2,137 nasal, conjunctival, and rectal swabs as well as whole blood and lung samples of pigs were collected in 19 regions from ten provinces of China in this study.ResultsWe report an overall positivity of 62.4% (1,334/2,137) of C. suis following screening by Chlamydia spp. 23S rRNA-based FRET-PCR and high-resolution melting curve analysis and confirmatory sequencing. For C. suis-positive samples, 33.3 % of whole blood and 62.5% of rectal swabs were found to be positive for the C. suis tetR(C) gene, while 13.3% of whole blood and 87.0% of rectal swabs were positive for the C. suis tet(C) gene. Phylogenetic comparison of partial C. suis ompA gene sequences revealed significant genetic diversity in the C. suis strains. This genetic diversity was confirmed by C. suis-specific multilocus sequence typing (MLST), which identified 26 novel sequence types among 27 examined strains. Tanglegrams based on MLST and ompA sequences provided evidence of C. suis recombination amongst the strains analyzed.ConclusionsGenetically highly diverse C. suis strains are exceedingly prevalent in pigs. As it stands, the potential pathogenic effect of C. suis on pig health and production of C. suis remains unclear and will be the subject of further investigations. Further study is also required to address the transmission of C. suis between pigs and the risk of 'spill-over' and 'spill-back' of infections to wild animals and humans.

【 授权许可】

CC BY   
© The Author(s). 2017